User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/24

(Difference between revisions)

Revision as of 13:21, 24 October 2012

Project name

The PCR product was transformed by following the Transformation Protocol
ADA protein was expressed in the same manner as on 2012/09/19
- 5mL of .4M IPTG solution was made and 1mL was placed into each of the 4 fernbach flasks.
- .005L x.4M= .002 moles x 238.31g/mol= .477g IPTG in 5mL. Actual= .4754g in 5mL of water.

For information regarding the Lysozyme please see either mikes notebook or marys notebook.

@@ Line 6: / Line 6: @@
 | colspan="2"|
 <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-==Entry title==
+==Transformation, protein expression, lysozyme Uv-Vis==
-* Insert content here...
+==Goals==
+*Transform PCR product into E.Coli Cells
+* Induce ADA protein expression
+*Run Uv-Vis of the lysozyme and AuNP solution
+==Procedure==
+* The PCR product was transformed by following the [[AU Biomaterials Design Lab:Protocols/Transformation Protocol| Transformation Protocol]]
+* ADA protein was expressed in the same manner as on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/19| 2012/09/19]]
+** 5mL of .4M IPTG solution was made and 1mL was placed into each of the 4 fernbach flasks.
+** .005L x.4M= .002 moles x 238.31g/mol= .477g IPTG in 5mL. Actual= .4754g in 5mL of water.
+* For information regarding the Lysozyme please see either mikes notebook or marys notebook.