User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/24

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(Autocreate 2012/10/24 Entry for User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles)
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==Entry title==
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==Transformation, protein expression, lysozyme Uv-Vis==
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* Insert content here...
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==Goals==
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*Transform PCR product into E.Coli Cells
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* Induce ADA protein expression
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*Run Uv-Vis of the lysozyme and AuNP solution
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==Procedure==
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* The PCR product was transformed by following the [[AU Biomaterials Design Lab:Protocols/Transformation Protocol| Transformation Protocol]]
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* ADA protein was expressed in the same manner as on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/19| 2012/09/19]]
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** 5mL of .4M IPTG solution was made and 1mL was placed into each of the 4 fernbach flasks.  
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** .005L x.4M= .002 moles x 238.31g/mol= .477g IPTG in 5mL. Actual= .4754g in 5mL of water.
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* For information regarding the Lysozyme please see either mikes notebook or marys notebook.
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Revision as of 12:21, 24 October 2012

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Transformation, protein expression, lysozyme Uv-Vis

Goals

  • Transform PCR product into E.Coli Cells
  • Induce ADA protein expression
  • Run Uv-Vis of the lysozyme and AuNP solution

Procedure

  • The PCR product was transformed by following the Transformation Protocol
  • ADA protein was expressed in the same manner as on 2012/09/19
    • 5mL of .4M IPTG solution was made and 1mL was placed into each of the 4 fernbach flasks.
    • .005L x.4M= .002 moles x 238.31g/mol= .477g IPTG in 5mL. Actual= .4754g in 5mL of water.
  • For information regarding the Lysozyme please see either mikes notebook or marys notebook.




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