User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/24: Difference between revisions
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== | ==Transformation, protein expression, lysozyme Uv-Vis== | ||
* | |||
==Goals== | |||
*Transform PCR product into E.Coli Cells | |||
* Induce ADA protein expression | |||
*Run Uv-Vis of the lysozyme and AuNP solution | |||
==Procedure== | |||
* The PCR product was transformed by following the [[AU Biomaterials Design Lab:Protocols/Transformation Protocol| Transformation Protocol]] | |||
* ADA protein was expressed in the same manner as on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/19| 2012/09/19]] | |||
** 5mL of .4M IPTG solution was made and 1mL was placed into each of the 4 fernbach flasks. | |||
** .005L x.4M= .002 moles x 238.31g/mol= .477g IPTG in 5mL. Actual= .4754g in 5mL of water. | |||
* For information regarding the Lysozyme please see either mikes notebook or marys notebook. | |||
Revision as of 10:21, 24 October 2012
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Transformation, protein expression, lysozyme Uv-VisGoals
Procedure
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