User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/24: Difference between revisions
From OpenWetWare
Line 22: | Line 22: | ||
* ADA protein was expressed in the same manner as on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/19| 2012/09/19]] | * ADA protein was expressed in the same manner as on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/19| 2012/09/19]] | ||
** This morning, Michael came in around 9am to centrifuge the starter culture media and resuspended the pellets in 4mL of fresh LB. These resuspended cells were then redivided into the expression culture media which was then inoculated in the shaker at 160rpm and 37°C. Finally, the IPTG was added at around 10am. | |||
** 5mL of .4M IPTG solution was made and 1mL was placed into each of the 4 fernbach flasks. | ** 5mL of .4M IPTG solution was made and 1mL was placed into each of the 4 fernbach flasks. | ||
** .005L x.4M= .002 moles x 238.31g/mol= .477g IPTG in 5mL. Actual= .4754g in 5mL of water. | ** .005L x.4M= .002 moles x 238.31g/mol= .477g IPTG in 5mL. Actual= .4754g in 5mL of water. | ||
* The 4 flasks were then shaken for 4 hours. | |||
* For information regarding the Lysozyme please see either [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/24| Mikes notebook]] or [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/24| Marys notebook]]. The lysozyme did create purple gold nanoparticle fibers upon removal from the oven. | * For information regarding the Lysozyme please see either [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/24| Mikes notebook]] or [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/24| Marys notebook]]. The lysozyme did create purple gold nanoparticle fibers upon removal from the oven. |
Revision as of 17:16, 21 November 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Transformation, protein expression, lysozyme Uv-VisGoals
Procedure
|