User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/23

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(Autocreate 2012/10/23 Entry for User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles)
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==Entry title==
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==More ADA and AAS==
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* Insert content here...
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==Goals==
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* Run AAS from Au/BSA solutions
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* Make starter culture media and expression culture media
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==Procedure==
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* For the Starter culture media and expression culture media, the same procedure from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18| September 18]]
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* The samples of broth were made and autoclaved today. The antibiotic and the E.coli colony were also added into the starter culture media.
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==Data==
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Starter Culture Media-- amount of LB added to each flask
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# Flask 1: 25.0095g
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# Flask 2: 25.0059g
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# Flask 3: 25.009g
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# Flask 4: 25.0008g
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Expression Culture Media:
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# Flask 1: .8744g
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# Flask 2: .8775g
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# Flask 3: .8745g
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# Flask 4L .8749g

Revision as of 13:17, 23 October 2012

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More ADA and AAS

Goals

  • Run AAS from Au/BSA solutions
  • Make starter culture media and expression culture media

Procedure

  • For the Starter culture media and expression culture media, the same procedure from September 18
  • The samples of broth were made and autoclaved today. The antibiotic and the E.coli colony were also added into the starter culture media.

Data

Starter Culture Media-- amount of LB added to each flask

  1. Flask 1: 25.0095g
  2. Flask 2: 25.0059g
  3. Flask 3: 25.009g
  4. Flask 4: 25.0008g

Expression Culture Media:

  1. Flask 1: .8744g
  2. Flask 2: .8775g
  3. Flask 3: .8745g
  4. Flask 4L .8749g



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