User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/17: Difference between revisions
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==Procedure== | ==Procedure== | ||
*Two solutions were created. In each one, 1μL of DPN1 was added to 45μL K110A (f) and K110A (r) plasmid respectively. These plasmid solutions were the same ones made and placed in the thermocycler [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/16| Yesterday]] | *Two solutions were created. In each one, 1μL of DPN1 was added to 45μL K110A (f) and K110A (r) plasmid respectively. These plasmid solutions were the same ones made and placed in the thermocycler [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/16| Yesterday]]. The two new solutions were then placed on a heat block at 37°C for 60 minutes. | ||
*Next, 5μL of the two solutions K110A (f) and K110A (r) plasmids were combined with 1μL of 6X Gel Loading Dye (blue). the 6μL solutions were then loaded into an agarose gel for gel electrophoresis. | *Next, 5μL of the two solutions K110A (f) and K110A (r) plasmids were combined with 1μL of 6X Gel Loading Dye (blue). the 6μL solutions were then loaded into an agarose gel for gel electrophoresis. | ||
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As I was not present for the next segment of the experiment, these steps and information was taken from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/17| Keyun Wang's Notebook]] | As I was not present for the next segment of the experiment, these steps and information was taken from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/17| Keyun Wang's Notebook]] | ||
* | * "Gel was stained in TAE buffer with ethidium bromide. The gel, as well as 20uL of 12.3mg/mL ethidium bromide were added into 100mL of TAE buffer and mixed for 5 minutes. Gel was then transferred to TAE buffer without ethidium bromide to rinse for 5 minutes." | ||
* Gel was observed under UV lamp to see if PCR product has been successfully produced. | * "Gel was observed under UV lamp to see if PCR product has been successfully produced." | ||
==Conclusions== | ==Conclusions== |
Revision as of 16:46, 21 November 2012
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Gel ElectrophoresisGoals
Procedure
As I was not present for the next segment of the experiment, these steps and information was taken from Keyun Wang's Notebook
Conclusions
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