User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/17: Difference between revisions
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**1.23 grams of agarose was mixed with 35mL of 1X TAE buffer (buffer made of Tris base, Acetic acid and Ethylenediaminetetraacetic acid (EDTA) and then microwaved for 45 seconds. This solution was then quickly poured into the Gel electrophoresis and was given approximately 20-30 minutes to solidify. 1X TAE buffer was then poured into the gel electrophoresis container. | **1.23 grams of agarose was mixed with 35mL of 1X TAE buffer (buffer made of Tris base, Acetic acid and Ethylenediaminetetraacetic acid (EDTA) and then microwaved for 45 seconds. This solution was then quickly poured into the Gel electrophoresis and was given approximately 20-30 minutes to solidify. 1X TAE buffer was then poured into the gel electrophoresis container. | ||
** A DNA ladder was made by mixing 5uL DNA ladder with 1uL of 6X gel loading dye blue. The Plasmids with different ADA mutations were then loaded in the gel. There are a total of 3 different plasmids each one with a f (forward) and an r (reverse). Therefore, between the entire lab, there were 6 plasmids. They were loaded in gel in the following order: | ** A DNA ladder was made by mixing 5uL DNA ladder with 1uL of 6X gel loading dye blue. The Plasmids with different ADA mutations were then loaded in the gel. There are a total of 3 different plasmids each one with a f (forward) and an r (reverse). Therefore, between the entire lab, there were 6 plasmids. They were loaded in gel in the following order: | ||
***DNA ladder, E34K, E34A , E34A, K110A, K110A, E34K | |||
** The Gel electrophoresis was run at 85 volts for 1 hour. | ** The Gel electrophoresis was run at 85 volts for 1 hour. | ||
Revision as of 12:56, 24 October 2012
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Gel ElectrophoresisGoals
Procedure
As I was not present for the next segment of the experiment, these steps and information was taken from Keyun Wang's Notebook
Conclusions
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