User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/16

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

PCR of ADA Mutations

Goals

  • Prepare ADA primers in order to conduct a polymerase chain reaction (PCR)
  • Run HRP Luminol Assay

Procedure

  • Assigned Primer= ADA K110A F (forward primer)
    • Sequence Issue number: 87635082
    • Sequence: ATT TGG CCA AAT GGG CAG TTA TTG AA
    • TM= 65.5°C
    • MW= 16833.0 g/mol
    • 33.4 nm= .56 mg ( amount of present plasmid)

concentration of plasmid needed for experiment= 100ng/μL

.56mg= 5600 ng

M1V1= M2V2 5600 ng/μL x V1= 100ng x 1000μL

  • (The container containing the plasmid only had room for 1mL of water to be added- as a result, a dilution needed to be done in order to get the appropriate concentration.

V2= 17.857μL is the amount of plasmid solution pipetted out in order to get a concentration of 100ng/μL


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/10/16&oldid=640237"