User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/16: Difference between revisions
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* a new stock of luminol solution was made at a pH of 10.56. For full details on preparation, calculation, and concentration please see [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16| Mary's Notebook]] | * a new stock of luminol solution was made at a pH of 10.56. For full details on preparation, calculation, and concentration please see [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16| Mary's Notebook]] | ||
* The other reagents were either left over from the HRP absorbance assay from [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/02| another week]] or were remade in using the same method. | |||
==Calculations== | |||
*Concentrations of the stock solutions: | |||
**10mM iodophenol in Dimethyl Sulfoxide (DMSO) | |||
**1.7mM H<sub>2</sub>0<sub>2</sub> | |||
**30mM luminol | |||
**1mg/mL HRP | |||
Total Volume in cuvet= 1500μL | |||
==Conclusions== | ==Conclusions== |
Revision as of 18:19, 23 October 2012
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PCR of ADA MutationsGoals
Preparation
Concentration of plasmid needed for experiment= 100ng/μL
.56mg= 5600 ng M1V1= M2V2 5600 ng/μL x V1= 100ng x 1000μL
V2= 17.857μL is the amount of plasmid solution pipetted out in order to get a concentration of 100ng/μL Procedure
Calculations
Total Volume in cuvet= 1500μL Conclusions
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