User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/16: Difference between revisions
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== | ==PCR of ADA Mutations== | ||
==Goals== | |||
*Prepare ADA primers in order to conduct a polymerase chain reaction (PCR) | |||
* Run HRP Luminol Assay | |||
==Procedure== | |||
* Assigned Primer= ADA K110A F (forward primer) | |||
** Sequence Issue number: 87635082 | |||
** Sequence: ATT TGG CCA AAT GGG CAG TTA TTG AA | |||
** TM= 65.5°C | |||
** MW= 16833.0 g/mol | |||
**33.4 nm= .56 mg ( amount of present plasmid) | |||
concentration of plasmid needed for experiment= 100ng/μL | |||
.56mg= 5600 ng | |||
M<sub>1</sub>V<sub>1</sub>= M<sub>2</sub>V<sub>2</sub> | |||
5600 ng/μL * V<sub>1</sub>= 100ng * 1000μL | |||
**(The container containing the plasmid only had room for 1mL of water to be added- as a result, a dilution needed to be done in order to get the appropriate concentration. | |||
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Revision as of 17:50, 23 October 2012
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PCR of ADA MutationsGoals
Procedure
concentration of plasmid needed for experiment= 100ng/μL .56mg= 5600 ng M1V1= M2V2 5600 ng/μL * V1= 100ng * 1000μL
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