User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/16
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| - | == | + | ==PCR of ADA Mutations== |
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| + | ==Goals== | ||
| + | *Prepare ADA primers in order to conduct a polymerase chain reaction (PCR) | ||
| + | * Run HRP Luminol Assay | ||
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| + | ==Procedure== | ||
| + | * Assigned Primer= ADA K110A F (forward primer) | ||
| + | ** Sequence Issue number: 87635082 | ||
| + | ** Sequence: ATT TGG CCA AAT GGG CAG TTA TTG AA | ||
| + | ** TM= 65.5°C | ||
| + | ** MW= 16833.0 g/mol | ||
| + | **33.4 nm= .56 mg ( amount of present plasmid) | ||
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| + | concentration of plasmid needed for experiment= 100ng/μL | ||
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| + | .56mg= 5600 ng | ||
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| + | M<sub>1</sub>V<sub>1</sub>= M<sub>2</sub>V<sub>2</sub> | ||
| + | 5600 ng/μL * V<sub>1</sub>= 100ng * 1000μL | ||
| + | **(The container containing the plasmid only had room for 1mL of water to be added- as a result, a dilution needed to be done in order to get the appropriate concentration. | ||
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Revision as of 20:50, 23 October 2012
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PCR of ADA MutationsGoals
Procedure
concentration of plasmid needed for experiment= 100ng/μL .56mg= 5600 ng M1V1= M2V2 5600 ng/μL * V1= 100ng * 1000μL
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