User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/16

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(Autocreate 2012/10/16 Entry for User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles)
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==Entry title==
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==PCR of ADA Mutations==
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* Insert content here...
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==Goals==
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*Prepare ADA primers in order to conduct a polymerase chain reaction (PCR)
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* Run HRP Luminol Assay
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==Procedure==
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* Assigned Primer= ADA K110A F (forward primer)
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** Sequence Issue number: 87635082
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** Sequence: ATT TGG CCA AAT GGG CAG TTA TTG AA
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** TM= 65.5°C
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** MW= 16833.0 g/mol
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**33.4 nm= .56 mg ( amount of present plasmid)
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concentration of plasmid needed for experiment= 100ng/μL
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.56mg= 5600 ng
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M<sub>1</sub>V<sub>1</sub>= M<sub>2</sub>V<sub>2</sub>
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5600 ng/μL * V<sub>1</sub>= 100ng * 1000μL 
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**(The container containing the plasmid only had room for 1mL of water to be added- as a result, a dilution needed to be done in order to get the appropriate concentration.
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Revision as of 20:50, 23 October 2012

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PCR of ADA Mutations

Goals

  • Prepare ADA primers in order to conduct a polymerase chain reaction (PCR)
  • Run HRP Luminol Assay

Procedure

  • Assigned Primer= ADA K110A F (forward primer)
    • Sequence Issue number: 87635082
    • Sequence: ATT TGG CCA AAT GGG CAG TTA TTG AA
    • TM= 65.5°C
    • MW= 16833.0 g/mol
    • 33.4 nm= .56 mg ( amount of present plasmid)

concentration of plasmid needed for experiment= 100ng/μL

.56mg= 5600 ng

M1V1= M2V2 5600 ng/μL * V1= 100ng * 1000μL

    • (The container containing the plasmid only had room for 1mL of water to be added- as a result, a dilution needed to be done in order to get the appropriate concentration.


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