User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/09

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(Data/Results)
Current revision (00:59, 7 December 2012) (view source)
(Procedure)
 
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==Procedure==
==Procedure==
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* The AA was calibrated using standard solutions made by [[User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08| Dr. Miller]]. The concentrations of these standard solutions were 5,8,10,15,20,25,30 and 40 ppm. The test was also blanked with 1M HCl.  
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* The AA was calibrated using standard solutions made by [[User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08| Dr. Miller]]. The concentrations of these standard solutions were 5,8,10,15,20,25,30 and 40 ppm. The AA runs were blanked with 1M HCl.  
*Deionized water was run through the AA between samples.  
*Deionized water was run through the AA between samples.  
* The Au/BSA samples had been made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]].  
* The Au/BSA samples had been made on [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/26| 2012/09/26]].  
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*The samples were runt through the AA and blanked with 1M HCl between every 4 samples.  
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**Fluid was removed from fibers before being analyzed, as fibers could clog the tubing.
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*The samples were runt through the AA and blanked with 1M HCl between every 4 samples.
==Data/Results==
==Data/Results==
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| 170||29.3405||0.5622
| 170||29.3405||0.5622
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* Absorbance and concentrations of standards and solutions. It can be seen that there is a general trend. as concentration increases, so does absorbance. There is a drop and then a spike and the 138-140 range.
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[[Image:AASRound1.png]]
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[[Image:AA calibration cuve.png| AA calibration curves]]
==Conclusions==
==Conclusions==
*For unknown reasons, there were no fibers present in the solution. But it was apparent that the gold had reacted because the solution was purple. Therefore, we went ahead with the experiment with the intent or repeating it at a later date.  
*For unknown reasons, there were no fibers present in the solution. But it was apparent that the gold had reacted because the solution was purple. Therefore, we went ahead with the experiment with the intent or repeating it at a later date.  
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* The AAS allowed us to conclude that the measured concentration of gold in the solution was different from what we had anticipated when we made the solutions at specific mole ratios. This could indicate the fact that the lack of NP fibers in these samples resulted in the variation in concentration observed.

Current revision

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Atomic Absorption Spectrum

Goals

  • Run AAS on AuNP/BSA samples to determine true concentrations

Procedure

  • The AA was calibrated using standard solutions made by Dr. Miller. The concentrations of these standard solutions were 5,8,10,15,20,25,30 and 40 ppm. The AA runs were blanked with 1M HCl.
  • Deionized water was run through the AA between samples.
  • The Au/BSA samples had been made on 2012/09/26.
    • Fluid was removed from fibers before being analyzed, as fibers could clog the tubing.
  • The samples were runt through the AA and blanked with 1M HCl between every 4 samples.

Data/Results

Standard True Value Concentration Absorbance
5-0.0913
8-0.1547
10-0.1993
15-0.3175
20-0.4059
25-0.4918
30-0.5928
40-0.7299
Blank-0.0198


Sample Concentration Absorbance
6011.09720.2228
8015.39730.3028
10021.62710.4187
12021.76690.4213
12822.44420.4339
13023.77180.4586
13221.35840.4137
13323.05150.4452
13419.14920.3726
13625.36290.4882
13824.63180.4746
14024.21260.4668
16027.26030.5235
17029.34050.5622


  • Absorbance and concentrations of standards and solutions. It can be seen that there is a general trend. as concentration increases, so does absorbance. There is a drop and then a spike and the 138-140 range.

Image:AASRound1.png


AA calibration curves

Conclusions

  • For unknown reasons, there were no fibers present in the solution. But it was apparent that the gold had reacted because the solution was purple. Therefore, we went ahead with the experiment with the intent or repeating it at a later date.
  • The AAS allowed us to conclude that the measured concentration of gold in the solution was different from what we had anticipated when we made the solutions at specific mole ratios. This could indicate the fact that the lack of NP fibers in these samples resulted in the variation in concentration observed.



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