User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/03

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Kinetics

Goals

  • Run UV-Vis of Absorbance vs. time of the HRP assay.

Procedure

  • We ran multiple UV-Vis spectras of the HRP with the gold nanoparticles to examine the impact that the NPs had on the kinetics of the reaction.

Data

  • We ran 5 main tests and several of the tests multiple times to confirm the data. The multiple tests showed that the experiment was not really reproducible because we seemed to be getting different spectras every time.
Trial run A ' ' ' ' ' '
Cuvethydrogen peroxide concentration (mM)Iodophenol in DMSO concentration (μM)AAP Concentration (μM)HRP concentration (μM)μL of gold np addedμL of water added
1( standard)1.718156.252.30630
11.718156.252.320610
21.718156.252.340590
31.718156.252.360570
41.718156.252.380550
51.718156.252.3100530
Total volume added750 μL10 μL35 μL22 μL

The amount of HRP was changed from 22μL to 33μL because the 22μL in the cuvet did not give a linear spectra, just a horizontal line. More was needed in order to really push the reaction.



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