User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/03 - Revision history

Puja Mody: /* Conclusions */

2012-10-23T18:58:25Z

Conclusions

←Older revision		Revision as of 18:58, 23 October 2012
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	==Conclusions==		==Conclusions==
-	~~Graphs of the data can be found on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\| Mary's notebook]].~~	+

2012-10-23T18:51:03Z

Conclusions

←Older revision		Revision as of 18:51, 23 October 2012
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	==Conclusions==		==Conclusions==
-	Graphs of the data ~~should~~ be found on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\| Mary's notebook]] ~~and analysis of data and rate reactions will be on in the future~~.	+	Graphs of the data can be found on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\| Mary's notebook]].
-		+

2012-10-23T18:48:54Z

←Older revision		Revision as of 18:48, 23 October 2012
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	==Goals==		==Goals==
-	* ~~Run UV-Vis of Absorbance vs. time of the HRP assay.~~	+	* Test activity of HRP int he presence of AuNP by using a Uv-Vis Absorbance HRP assay.
-		+
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 11:11, 7 October 2012 (EDT)''': that is what you did to accomplish your goal. yor goal is to test the activity of HRP ~~in the~~ presence of ~~AuNPs~~ by using a Uv-Vis Absorbance HRP assay.	+

	==Procedure==		==Procedure==
-	* We ran multiple UV-Vis spectras of the HRP with the gold nanoparticles to examine the impact that the NPs had on the kinetics of the reaction. We ran each run for 10 minutes and collected the various spectras.	+	* We ran multiple UV-Vis spectras of the HRP with the gold nanoparticles to examine the impact that the NPs had on the kinetics of the reaction. We ran each run for 10 minutes and collected the various spectras. We maintained the same concentration in the cuvet of all the reagents except for the concentration of the gold nanoparticles. As a result, we were examining the effect that gold nanoparticles had on the rate of the reaction.

	==Data==		==Data==

2012-10-23T18:46:38Z

Procedure

←Older revision		Revision as of 18:46, 23 October 2012
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	==Procedure==		==Procedure==
-	* We ran multiple UV-Vis spectras of the HRP with the gold nanoparticles to examine the impact that the NPs had on the kinetics of the reaction.	+	* We ran multiple UV-Vis spectras of the HRP with the gold nanoparticles to examine the impact that the NPs had on the kinetics of the reaction. We ran each run for 10 minutes and collected the various spectras.
-		+
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 11:13, 7 October 2012 (EDT)''': this needs to tell me exactly what you did. what solutions you mixed? how long did it take? what concentrations? what were the parameters for the ~~Uv-VIs? what do you need to know to exactly reproduce this from your notebook only~~.	+

	==Data==		==Data==

2012-10-23T18:45:42Z

Conclusions

←Older revision		Revision as of 18:45, 23 October 2012
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	==Conclusions==		==Conclusions==
-	Graphs of the data should be found on [[User:~~Mary_Mendoza~~/Notebook/~~CHEM_571_Experimental_Biological_Chemistry_I~~/2012/10/03\| Mary's notebook]] and analysis of data and rate reactions will be on in the future.	+	Graphs of the data should be found on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\| Mary's notebook]] and analysis of data and rate reactions will be on in the future.
		+

-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 11:14, 7 October 2012 (EDT)''':you need to check that before you make the link back to someone else's notebook..

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

2012-10-07T15:14:42Z

Conclusions

←Older revision		Revision as of 15:14, 7 October 2012
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	==Conclusions==		==Conclusions==
	Graphs of the data should be found on [[User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/10/03\| Mary's notebook]] and analysis of data and rate reactions will be on in the future.		Graphs of the data should be found on [[User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/10/03\| Mary's notebook]] and analysis of data and rate reactions will be on in the future.
		+
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 11:14, 7 October 2012 (EDT)''':you need to check that before you make the link back to someone else's notebook..

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

2012-10-07T15:13:16Z

Procedure

←Older revision		Revision as of 15:13, 7 October 2012
Line 15:		Line 15:

	==Procedure==		==Procedure==
-	* We ran multiple UV-Vis spectras of the HRP with the gold nanoparticles to examine the impact that the NPs had on the kinetics of the reaction.	+	* We ran multiple UV-Vis spectras of the HRP with the gold nanoparticles to examine the impact that the NPs had on the kinetics of the reaction.
		+
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 11:13, 7 October 2012 (EDT)''': this needs to tell me exactly what you did. what solutions you mixed? how long did it take? what concentrations? what were the parameters for the Uv-VIs? what do you need to know to exactly reproduce this from your notebook only.

	==Data==		==Data==

2012-10-07T15:11:28Z

Goals

←Older revision		Revision as of 15:11, 7 October 2012
Line 10:		Line 10:

	==Goals==		==Goals==
-	* Run UV-Vis of Absorbance vs. time of the HRP assay.	+	* Run UV-Vis of Absorbance vs. time of the HRP assay.
		+
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 11:11, 7 October 2012 (EDT)''': that is what you did to accomplish your goal. yor goal is to test the activity of HRP in the presence of AuNPs by using a Uv-Vis Absorbance HRP assay.

	==Procedure==		==Procedure==

2012-10-05T17:47:22Z

Data

←Older revision		Revision as of 17:47, 5 October 2012
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	\| Total volume added\|\|750 μL\|\|10 μL\|\|35 μL\|\|22 μL\|\|\|\|		\| Total volume added\|\|750 μL\|\|10 μL\|\|35 μL\|\|22 μL\|\|\|\|
	\|}		\|}
		+

	The amount of HRP was changed from 22μL to 33μL because the 22μL in the cuvet did not give a linear spectra, just a horizontal line. More was needed in order to really push the reaction.		The amount of HRP was changed from 22μL to 33μL because the 22μL in the cuvet did not give a linear spectra, just a horizontal line. More was needed in order to really push the reaction.
		+
		+
		+	[[Image:HRP with AuNPs.png\| HRP with AuNPs]]

2012-10-05T16:06:56Z

Data

←Older revision		Revision as of 16:06, 5 October 2012
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-	[[~~http://openwetware.org/wiki/~~Image:HRPruns.xlsx\| HRP catalyst and gold nanoparticles measuring absorbance over time]]	+	[[Image:HRPruns.xlsx\| HRP catalyst and gold nanoparticles measuring absorbance over time]]

	==Conclusions==		==Conclusions==