User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/03: Difference between revisions
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==Goals== | ==Goals== | ||
* | * Test activity of HRP int he presence of AuNP by using a Uv-Vis Absorbance HRP assay. | ||
==Procedure== | ==Procedure== | ||
* We ran multiple UV-Vis spectras of the HRP with the gold nanoparticles to examine the impact that the NPs had on the kinetics of the reaction. | * We ran multiple UV-Vis spectras of the HRP with the gold nanoparticles to examine the impact that the NPs had on the kinetics of the reaction. We ran each run for 10 minutes and collected the various spectras. We maintained the same concentration in the cuvet of all the reagents except for the concentration of the gold nanoparticles. As a result, we were examining the effect that gold nanoparticles had on the rate of the reaction. | ||
==Data== | ==Data== | ||
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| Total volume added||750 μL||10 μL||35 μL||22 μL|||| | | Total volume added||750 μL||10 μL||35 μL||22 μL|||| | ||
|} | |} | ||
The amount of HRP was changed from 22μL to 33μL because the 22μL in the cuvet did not give a linear spectra, just a horizontal line. More was needed in order to really push the reaction. | The amount of HRP was changed from 22μL to 33μL because the 22μL in the cuvet did not give a linear spectra, just a horizontal line. More was needed in order to really push the reaction. | ||
[[Image:HRP with AuNPs.png| HRP with AuNPs]] | |||
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==Conclusions== | ==Conclusions== | ||
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Revision as of 11:58, 23 October 2012
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KineticsGoals
Procedure
Data
Conclusions |