User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/03: Difference between revisions
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==Data== | ==Data== | ||
* We ran 5 main tests and several of the tests multiple times to confirm the data. The multiple tests showed that the experiment was not really reproducible because we seemed to be getting different spectras every time. | |||
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| align="center" style="background:#f0f0f0;"|'''Trial run A''' | |||
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| Cuvet||hydrogen peroxide concentration (mM)||Iodophenol in DMSO concentration (μM)||AAP Concentration (μM)||HRP concentration (μM)||μL of gold np added||μL of water added | |||
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| 1( standard)||1.7||18||156.25||2.3||0||630 | |||
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| 1||1.7||18||156.25||2.3||20||610 | |||
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| 2||1.7||18||156.25||2.3||40||590 | |||
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| 3||1.7||18||156.25||2.3||60||570 | |||
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| 4||1.7||18||156.25||2.3||80||550 | |||
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| 5||1.7||18||156.25||2.3||100||530 | |||
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| Total volume added||750 μL||10 μL||35 μL||22 μL|||| | |||
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The amount of HRP was changed from 22μL to 33μL because the 22μL in the cuvet did not give a linear spectra, just a horizontal line. More was needed in order to really push the reaction. | |||
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Revision as of 21:57, 4 October 2012
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KineticsGoals
Procedure
Data
The amount of HRP was changed from 22μL to 33μL because the 22μL in the cuvet did not give a linear spectra, just a horizontal line. More was needed in order to really push the reaction.
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