User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/25: Difference between revisions
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*UV-Vis | *UV-Vis | ||
# New concentrations of the Tris buffer were added to the Gold/BSA solution. These solutions did not express any fibers, it was just purple solution. The UV-Vis was run twice on the samples with an hour in between each run. | # New concentrations of the Tris buffer were added to the Gold/BSA solution. These solutions did not express any fibers, it was just purple solution. The UV-Vis was run twice on the samples with an hour in between each run. | ||
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:56, 7 October 2012 (EDT)''':be careful. this sounds more like what you will do, not what you actually did. having both in your notebook is fine, but having the first only is not. | |||
==DATA/Calculations== | ==DATA/Calculations== |
Revision as of 07:56, 7 October 2012
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Separate ProteinsGoals
Procedure
DATA/Calculations
to make a 25mL stock solution of the tris buffer at a pH of 10 1mol/L x .025= .025 mol .025 mol x 121.14 g/mol = 3.0285 g of tris To get the necessary concentrations of tris into the gold/BSA solutions, the M1V1=M2V2 formula was used to calculate the volume of tris needed. From there, the value of the volume of tris was subtracted from 1 in order to determine the amount of water that would be added to make the desired concentration.
link for excel File:TrisvaryingmolarUVVis.xlsx Conclusions
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