User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/19: Difference between revisions
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==Protein Expression. == | ==Protein Expression. == | ||
* | * | ||
==Goals== | |||
*Induce Protein expression. | |||
* make the binding buffer and the elution buffer | |||
*Continue running AuNP tests- | |||
*Harvest the cells that were grown. | |||
==Procedure== | |||
*Protein Expression | |||
#This morning at 8 am, the starter cultures were centrifuged at 4500 rpm for 15 minutes and then resuspended the cell pellets in 4mL of fresh LB. | |||
# Inoculate the [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture media]] by dividing the resuspended cells among the Fernbach flasks. | |||
# Incubate the expression cultures at 37C and 160rpm until the absorbance of the media at 600nm = 0.6. | |||
# Add 1mL of 0.4M [[AU_Biomaterials_Design_Lab:Materials/IPTG|IPTG]] to each flask in order to induce protein expression | |||
# Continue shaking for 3-4 hours | |||
# Harvest the cells by centrifuging at 4500rpm for 15 minutes | |||
==Conclusions== | |||
*sample 1 looked a little different when the samples were looked at this morning prior to centrifuging. It was a different shade of yellow in the erlenmeyer flask. | |||
Revision as of 12:26, 19 September 2012
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Protein Expression.Goals
Procedure
Conclusions
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