User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/19: Difference between revisions
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==Procedure== | ==Procedure== | ||
*Protein Expression | *Protein Expression | ||
#This morning at 8 am, the starter cultures were centrifuged at 4500 rpm for 15 minutes and then resuspended the cell pellets in 4mL of fresh LB. | #This morning at 8 am, the starter cultures were centrifuged at 4500 rpm for 15 minutes and then resuspended the cell pellets in 4mL of fresh LB. For more details on the process in the morning, see [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/19| Mary's Notebook]] | ||
# Inoculate the [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture media]] by dividing the resuspended cells among the Fernbach flasks. | # Inoculate the [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|expression culture media]] by dividing the resuspended cells among the Fernbach flasks. | ||
# Incubate the expression cultures at 37°C and 160 rpm until the absorbance of the media at 600nm = 0.6. | # Incubate the expression cultures at 37°C and 160 rpm until the absorbance of the media at 600nm = 0.6. |
Revision as of 23:42, 2 October 2012
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Protein Expression.Goals
Procedure
Calculations
a. Theoretical calculations
a. Theoretical calculations
Conclusions
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