User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18

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Day 5

Protein Expressions and Starters

Goals

Grow E.Coli cells and have them express ADA protein.
Follow Protocols for making starter and expression cultures of the cells.

Procedure

Starter Culture Protocol: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media
- add 35 mL to each to 4 250mL erlenmeyer flask

Expression culture Protocols: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media

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