User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18: Difference between revisions
Line 48: | Line 48: | ||
* Aseptic techniques were applied by executing the inoculation process near the proximity of a Bunsen burner. The aluminum foil tops of the Erlenmeyer containing the broths were flamed in between uncapping and capping. The metal tweezers were flamed before and after use. Sterile wood sticks were used to capture a colony of the ''E.Coli'' | * Aseptic techniques were applied by executing the inoculation process near the proximity of a Bunsen burner. The aluminum foil tops of the Erlenmeyer containing the broths were flamed in between uncapping and capping. The metal tweezers were flamed before and after use. Sterile wood sticks were used to capture a colony of the ''E.Coli'' | ||
*The starter culture media flasks were then placed in a shaker incubator at 37°C at 237rpm overnight. | |||
Preparation of Tris Buffer | |||
*pH 10- this buffer would be used with the AuNP/BSA made at a concentration of 70 [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/12| last week]] | |||
the desired concentration of the stock was .1 M tris and a total of 25mL of stock was made. | |||
1 mol/ 1L x .025 L= .025 mol | |||
.025 mol x 121.14 grams/1 mol = 3.0285 grams of tris in 25mL of water | |||
* in actuality, 3.080 grams of tris was added. | |||
Revision as of 23:31, 2 October 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Day 5
Goals
Procedure
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)
Using the formula M1V1=M2V2 The volume or amount of kanamycin that would need to go into each of the flasks was 50 μg/mL x 35mL ÷50,000 μg = .035mL Kanamycin.
Preparation of Tris Buffer
the desired concentration of the stock was .1 M tris and a total of 25mL of stock was made. 1 mol/ 1L x .025 L= .025 mol .025 mol x 121.14 grams/1 mol = 3.0285 grams of tris in 25mL of water * in actuality, 3.080 grams of tris was added.
|