User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18: Difference between revisions

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(Autocreate 2012/09/18 Entry for User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles)
 
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==Entry title==
==Day 5==
* Insert content here...
* Protein Expressions and Starters
 
==Goals==
*Grow E.Coli cells and have them express ADA protein.
*Follow Protocols for making starter and expression cultures of the cells.  
 
==Procedure==
* Starter Culture Protocol: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media
** add 35 mL to each to 4 250mL erlenmeyer flask
 
* Expression culture Protocols: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media





Revision as of 10:20, 18 September 2012

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Day 5

  • Protein Expressions and Starters

Goals

  • Grow E.Coli cells and have them express ADA protein.
  • Follow Protocols for making starter and expression cultures of the cells.

Procedure