User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18: Difference between revisions
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==Procedure== | ==Procedure== | ||
* [[http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media| Started Culture Protocol]] | * [[http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media| Started Culture Protocol]] | ||
** add 35 mL | ** add 35 mL H<sub>2</sub>Oto each to 4 250mL erlenmeyer flask | ||
** [[AU_Biomaterials_Design_Lab:Materials/LB|LB]] was added to each flask. | ** [[AU_Biomaterials_Design_Lab:Materials/LB|LB]] was added to each flask. | ||
*** Flask 1=.8793g | *** Flask 1=.8793g |
Revision as of 22:50, 2 October 2012
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Day 5
Goals
Procedure
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)
Using the formula M1V1=M2V2 The volume or amount of kanamycin that would need to go into each of the flasks was 50 μg/mL x 35mL ÷50,000 μg = .35mL Kanamycin.
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