User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18: Difference between revisions
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* in actuality, 3.080 grams of tris was added. | * in actuality, 3.080 grams of tris was added. | ||
Calculations for amount of water and tris needed to achieve desired concentrations: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Tris (mM)''' | |||
| align="center" style="background:#f0f0f0;"|'''mL of water''' | |||
| align="center" style="background:#f0f0f0;"|'''Amount of tris to be added (mL)''' | |||
|- | |||
| 0.05||0.994||0.00625 | |||
|- | |||
| 5||0.998||0.0125 | |||
|- | |||
| 5||0.975||0.025 | |||
|- | |||
| 50||0.95||0.05 | |||
|- | |||
| 100||0.9||0.1 | |||
|- | |||
| 200||0.8||0.2 | |||
|- | |||
| 500||0.5||0.5 | |||
|- | |||
| 0.1||0||1 | |||
|} | |||
(The tris and water were not combined with the AuNP/BSa solutions today) | |||
Revision as of 23:34, 2 October 2012
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Day 5
Goals
Procedure
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)
Using the formula M1V1=M2V2 The volume or amount of kanamycin that would need to go into each of the flasks was 50 μg/mL x 35mL ÷50,000 μg = .035mL Kanamycin.
Preparation of Tris Buffer
the desired concentration of the stock was .1 M tris and a total of 25mL of stock was made. 1 mol/ 1L x .025 L= .025 mol .025 mol x 121.14 grams/1 mol = 3.0285 grams of tris in 25mL of water * in actuality, 3.080 grams of tris was added.
(The tris and water were not combined with the AuNP/BSa solutions today)
|