User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18: Difference between revisions
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* Starter Culture Protocol: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media | * Starter Culture Protocol: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media | ||
** add 35 mL to each to 4 250mL erlenmeyer flask | ** add 35 mL to each to 4 250mL erlenmeyer flask | ||
** [[AU_Biomaterials_Design_Lab:Materials/LB|LB]] was added to each flask. | |||
*** Flask 1=.8793g | |||
***Flask 2=.8800g | |||
***Flask 3=.8748g | |||
*** Flask 4=.8757g | |||
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask) | |||
* Expression culture Protocols: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media | * Expression culture Protocols: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media | ||
** 1L of distilled water were added to 4.8L Fernbach flasks | |||
**LB was added to each flask | |||
***Flask 1= 25.0289g | |||
***Flask 2= 25.0069g | |||
***Flask 3= 25.0020g | |||
***Flask 4= 25.0036g | |||
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask) | |||
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Revision as of 10:48, 18 September 2012
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Day 5
Goals
Procedure
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)
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