User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18

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(Procedure)
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* Starter Culture Protocol: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media
* Starter Culture Protocol: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media
** add 35 mL to each to 4 250mL erlenmeyer flask
** add 35 mL to each to 4 250mL erlenmeyer flask
 +
** [[AU_Biomaterials_Design_Lab:Materials/LB|LB]] was added to each flask.
 +
*** Flask 1=.8793g
 +
***Flask 2=.8800g
 +
***Flask 3=.8748g
 +
*** Flask 4=.8757g
 +
 +
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)
* Expression culture Protocols: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media
* Expression culture Protocols: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media
 +
** 1L of distilled water were added to 4.8L Fernbach flasks
 +
**LB was added to each flask
 +
***Flask 1= 25.0289g
 +
***Flask 2= 25.0069g
 +
***Flask 3= 25.0020g
 +
***Flask 4= 25.0036g
 +
 +
(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)
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Revision as of 13:48, 18 September 2012

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Day 5

  • Protein Expressions and Starters

Goals

  • Grow E.Coli cells and have them express ADA protein.
  • Follow Protocols for making starter and expression cultures of the cells.

Procedure

(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)


(note that while there were the amounts measured out- the amount of LB added to the flasks was actually less due to loss of sample in transferring it from the weigh dish to the flask)


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