User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/18
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| - | == | + | ==Day 5== |
| - | * | + | * Protein Expressions and Starters |
| + | |||
| + | ==Goals== | ||
| + | *Grow E.Coli cells and have them express ADA protein. | ||
| + | *Follow Protocols for making starter and expression cultures of the cells. | ||
| + | |||
| + | ==Procedure== | ||
| + | * Starter Culture Protocol: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media | ||
| + | ** add 35 mL to each to 4 250mL erlenmeyer flask | ||
| + | |||
| + | * Expression culture Protocols: http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media | ||
Revision as of 13:20, 18 September 2012
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