User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/05: Difference between revisions
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==Procedure== | ==Procedure== | ||
* The AuNP stock solution from yesterday was combined with a new stock solution of BSA. The concentrations that were retested were: 120, 128, 130, 132, 133, 134. These samples were then placed in the oven for four hours in order to allow for the BSA to unfold and to be wrapped around the AuNP. | * The AuNP stock solution from yesterday was combined with a new stock solution of BSA. The concentrations that were retested were: 120, 128, 130, 132, 133, 134. These samples were then placed in the oven for four hours in order to allow for the BSA to unfold and to be wrapped around the AuNP. These solutions were made by Dr. Miller on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/08/31| Friday]]. See her notebook for mass, volume and concentration values. | ||
* The AuNP solutions that has been used [[User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/09/04| previously]] were meanwhile transferred from glass test tubes to plastic ones in order to prepare them for centrifuging. The centrifuging was necessary in order to separate out the fibers as best as possible from the solution. | |||
* The AuNP solutions that has been used previously were meanwhile transferred from glass test tubes to plastic ones in order to prepare them for centrifuging. The centrifuging was necessary in order to separate out the fibers as best as possible from the solution | * The AuNP fibers were centrifuged for five minutes at a speed of 3000 rpm at a temperature of 10°C. * After centrifuging, the AuNP fibers were separated from solution by pipetting out as much of the supernatant as possible prior to adding the tris buffer. 7 test tubes which had centrifuged out the most of the AuNP fibers were used for the next step. Because it was the amount of Tris buffer that was being varied, the specific 7 test tues were not recorded. | ||
* A serial dilation was used in order to place various concentrations of the tris buffer and water into the test tubes. The concentrations ranged from .1M to 1×10<sup>−2</sup> to 1×10<sup>-7</sup>. We made 100mL of tris buffer at a concentration of .1M. We made the solutions by serial dilution directly in the test tubes with the fibers but were careful to not pipette out any of the fibers as we were doing the dilution. | |||
* The AuNP fibers were centrifuged for five minutes at a speed of 3000 rpm at a temperature of 10°C. After | * After these new solutions were made, they were placed in UV-Vis two times. each time to test if the fibers had been dissolved into the tris buffer solution. The first time point was immediately after the buffer and fibers were combined and the second run was 1 hours later. | ||
* A serial dilation was used in order to place various concentrations of the tris buffer and water into the test tubes. The concentrations ranged from .1M to 1×10<sup>−2</sup> to 1×10<sup>-7</sup>. | |||
* After these new solutions were made, they were placed in UV-Vis two times. each time to test if the fibers had been dissolved into the tris buffer solution. | |||
==Calculation== | ==Calculation== |
Revision as of 20:27, 2 October 2012
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Day 3Goals
Procedure
Calculation
(HAuCl4/BSA) 120, 128, 130, 132, 133, and 134. Volume of HAuCl4 stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of HAuCl4 stock solution and BSA stock solution from 6mL.
solution) = (1.5*6)
solution in each tube
stock solution
stock solution
stock solution
stock solution
stock solution
stock solution
Data
New concentrations of remixed AuNP/ BSA samples for UV-Vis
Conclusions
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