User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/05: Difference between revisions

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==Conclusions==
==Conclusions==
* The tris buffer solutions were placed in a UV-Vis a total of three times. for the data on those tests look at next entry.
* The tris buffer solutions were placed in a UV-Vis a total of three times. The first two tests were 1 hours apart. The Final trial was done on September 11.
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 13:43, 17 September 2012 (EDT)''':was absorbance measured on this date? if so you need to include the data here.

Revision as of 20:03, 2 October 2012

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Day 3

Goals

  • Remake certain AuNP/BSA solutions at specific concentrations to retest using UV-Vis to check/clarify discrepancies
  • Abigail E. Miller 13:43, 17 September 2012 (EDT):why are you retesting them?
  • Centrifuge the gold solutions in order to separate the fibers and then combine the fibers with the Tris buffer at various concentrations in order to test how long it takes to (if it does) re-suspend in the solution.
  • Abigail E. Miller 13:43, 17 September 2012 (EDT):resuspend what?

Procedure

  • The AuNP stock solution from yesterday was combined with a new stock solution of BSA. The concentrations that were retested were: 120, 128, 130, 132, 133, 134. These samples were then placed in the oven for four hours in order to allow for the BSA to unfold and to be wrapped around the AuNP.
  • Abigail E. Miller 13:43, 17 September 2012 (EDT):where did the stock solution come from? what was the mass and volume and concentration of it?
  • The AuNP solutions that has been used previously were meanwhile transferred from glass test tubes to plastic ones in order to prepare them for centrifuging. The centrifuging was necessary in order to separate out the fibers as best as possible from the solution.
  • Abigail E. Miller 13:43, 17 September 2012 (EDT):previosuly being what day? link to them.
  • The AuNP fibers were centrifuged for five minutes at a speed of 3000 rpm at a temperature of 10°C. After which, the BSA solution was pipetted out as best as was possible. 7 test tubes which had centrifuged out the most of the AuNP fibers were used for the next step.
  • Abigail E. Miller 13:43, 17 September 2012 (EDT):solution part after centrifuging is called the supernatant. which 7 fiber sampels were used?
  • A serial dilation was used in order to place various concentrations of the tris buffer and water into the test tubes. The concentrations ranged from .1M to 1×10−2 to 1×10-7.
  • Abigail E. Miller 13:43, 17 September 2012 (EDT):what is the stock Tris you started with? did you make the solutions by serial dilution then add to fibers?
  • After centrifuging, the AuNP fibers were separated from solution by pipetting out as much of the aqueous solution as possible prior to adding the tris buffer.
  • Abigail E. Miller 13:43, 17 September 2012 (EDT):list this before information about the bier resuspension.
  • After these new solutions were made, they were placed in UV-Vis two times. each time to test if the fibers had been dissolved into the tris buffer solution.
  • Abigail E. Miller 13:43, 17 September 2012 (EDT):what are the tiem points? at 30 min and 60 min ? or 30 and 120 min? or something different?

Calculation

  • Retested concentrations:
    1. Solutions were made with the mole ratios of

(HAuCl4/BSA) 120, 128, 130, 132, 133, and 134. Volume of HAuCl4 stock solution was calculated for each tube and inserted. The water needed for each tube was calculated by subtracting the volume of HAuCl4 stock solution and BSA stock solution from 6mL.

      1. m1v1=m2v2
      2. 15μM*(volume BSA stock

solution) = (1.5*6)

        1. 0.6mL BSA stock

solution in each tube

      1. 7520μM*x mL=6mL*(1.5*120μM)
        1. 0.143mL HAuCl4

stock solution

        1. 5.257mL H2O
      2. 7520μM*x mL=6mL*(1.5*128μM)
        1. 0.153mL HAuCl4

stock solution

        1. 5.247mL H2O
      2. 7520μM*x mL=6mL*(1.5*130μM)
        1. .155mL HAuCl4

stock solution

        1. 5.245mL H2O
      2. 7520μM*x mL=6mL*(1.5*132μM)
        1. 0.157mL HAuCl4

stock solution

        1. 5.243mL H2O
      2. 7520μM*x mL=6mL*(1.5*133μM)
        1. 0.159mL HAuCl4

stock solution

        1. 5.241mL H2O
      2. 7520μM*x mL=6mL*(1.5*134μM)
        1. 0.160mL HAuCl4

stock solution

        1. 5.240mL H2O

Data

Test Tube Tris Buffer (mL) Water (mL) Concentration of solution M
1 10 0 .1 M
1 1 9 1 x 10^-2 M
1 1 9 1 x 10^-3 M
1 1 9 1 x 10^-4 M
1 1 9 1 x10^-5 M
1 1 9 1 x 10 ^-6 M
1 1 9 1 x 10^-7 M


Links to spreadsheet and chart of redone UV-Vis:


New concentrations of remixed AuNP/ BSA samples for UV-Vis

Concentration mL of AuNP mL of BSA mL of H2O
120 0.144 0.6 5.256
128 0.156 0.6 5.247
130 0.155 0.6 5.245
132 0.158 0.6 5.242
133 0.159 0.6 5.241
134 0.16 0.6 5.24

Conclusions

  • The tris buffer solutions were placed in a UV-Vis a total of three times. The first two tests were 1 hours apart. The Final trial was done on September 11.
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