User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/08/29: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 10: Line 10:


==Goals==
==Goals==
* Make stock solutions of gold nanoparticles in water and BSA.
* Make stock solutions of gold nanoparticles (HAuCl<sub>4</sub> in water and BSA.
* Determine how much of each solution will be used in order to make the 14 different concentrations needed.  
* Determine how much of each solution will be used in order to make the 14 different concentrations needed.  


Line 17: Line 17:
* Next, a 25mL stock solution of BSA was made. The desired concentration of the BSA stock solution was 15μM. so .025×(15×10<sup>-6</sup>)= 3.75 x10<sup>-7</sup>μM.
* Next, a 25mL stock solution of BSA was made. The desired concentration of the BSA stock solution was 15μM. so .025×(15×10<sup>-6</sup>)= 3.75 x10<sup>-7</sup>μM.
.376 μM x66430 g/mol (MW of BSA)= .0249g BSA in 25mL of water.  
.376 μM x66430 g/mol (MW of BSA)= .0249g BSA in 25mL of water.  
* These concentrations were chosen based on the concentration ranges used in the experimentation by Bakshi.


==Results/Conclusions==
==Results/Conclusions==
* The actual concentration of the AuNP stock solution ended up being .00772 M/L due to the fact that some of the AuNP compound was lost on weigh paper when it was being weighed out and was therefore not transferred into the solution. The actual amount added into  the solution was .0656 grams HAuCl<sub>4</sub>  
* The actual concentration of the AuNP stock solution ended up being .00772 M/L due to the fact that some of the AuNP compound was lost on weigh paper when it was being weighed out and was therefore not transferred into the solution. The actual amount added into  the solution was .0656 grams HAuCl<sub>4</sub>.
.0656g x 1mol/339.79 (MW  HAuCl4)= 1.93 x10<sup>-4</sup> / .025L= .00772 M/L.
*A wide range of concentrations were then determined in order to best test the optimal range for the unfolding of the BSA protein and the insertion of the AuNP into the BSA. The concentrations chosen were 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, 170. This wide range was taken in order to determine exactly where optimal absorption of the AuNP into the BSA occurs.
 


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 19:13, 9 September 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Day 1

  • Insert content here...

Goals

  • Make stock solutions of gold nanoparticles (HAuCl4 in water and BSA.
  • Determine how much of each solution will be used in order to make the 14 different concentrations needed.

Procedure

  • A stock solution with the AuNP was first made with 25mL of water along with .085 grams of the gold nano- particles. This resulted in a stock solution concentration of 10mM.
  • Next, a 25mL stock solution of BSA was made. The desired concentration of the BSA stock solution was 15μM. so .025×(15×10-6)= 3.75 x10-7μM.

.376 μM x66430 g/mol (MW of BSA)= .0249g BSA in 25mL of water.

  • These concentrations were chosen based on the concentration ranges used in the experimentation by Bakshi.


Results/Conclusions

  • The actual concentration of the AuNP stock solution ended up being .00772 M/L due to the fact that some of the AuNP compound was lost on weigh paper when it was being weighed out and was therefore not transferred into the solution. The actual amount added into the solution was .0656 grams HAuCl4.

.0656g x 1mol/339.79 (MW HAuCl4)= 1.93 x10-4 / .025L= .00772 M/L.

  • A wide range of concentrations were then determined in order to best test the optimal range for the unfolding of the BSA protein and the insertion of the AuNP into the BSA. The concentrations chosen were 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, 170. This wide range was taken in order to determine exactly where optimal absorption of the AuNP into the BSA occurs.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Puja_Mody/Notebook/Chem_571:_Gold_Nanoparticles/2012/08/29&oldid=624592"