User:Puja Mody/Notebook/AU Biomaterials 572/2014/03/18

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==POST Spring Break: Day 12==
 
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*GOALS
 
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#Run AA on solutions prepared up to this point in order to determine the amount of Fe remaining in solution after synthesis of magnetic nanoparticles
 
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#Attempt preparation of magnetite nanoparticles with gold in order to produce fibers using a new protocol.
 
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==Procedure==
 
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'''AA standard Prep and data'''
 
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Please see [[User:Moira M. Esson/Notebook/CHEM-571/2014/03/18|Moira's Notebook]] for this procedure and the Data from the samples run.
 
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''Preparation of Magnetite nanoparticles with gold'''
 
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* The procedure used on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/03/05|3/05]] did not work and so we decided to modify the prep method in order to potentially produce fibers. We still maintained the method used on [[[[User:Puja Mody/Notebook/AU Biomaterials 572/2014/03/05|3/05]] for the beginning part of the synthesis procedure.
 
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*General Procedure
 
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# Prepare 5mL of reaction mixture for preparation of magnetite nanoparticles using the sample volumes and concentrations used on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11| 2/11]] ##We chose not to make all the ratio samples. And therefore picked the best 4 ratios. For BSA that was 13000:1 and 13500:1. And for Hemoglobin it was 16500:1 and 17000:1/
 
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#Cap the test tubes containing the sample with aluminum foil and place it in the oven at 80°C for 2 hours.
 
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#While magentite nanoparticles are heating, prepare 5mL of the equivalent ratio of gold:protein reaction mixture using the general protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/28|01/28/2014]].
 
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#After the 2 hours have completed, add 5mL of gold reaction mixture to the heated magnetite reaction mixture. Cap the tubes, place them back into the coven and continue to heat at 80°C for another 2 hours.
 
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<br>
 
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Table 1. Preparation of FeSO<sub>4</sub>*7H<sub>2</sub>O stock solution
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
 
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| align="center" style="background:#f0f0f0;"|''''''
 
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|-
 
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| Molecular Weight (g/mol)||278.01
 
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|-
 
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| mass(g)||0.83403
 
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|-
 
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| volume stock (L)||0.01
 
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|-
 
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| Concentration (M)||0.3
 
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|}
 
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<br>
 
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Table 2. Preparation of KNO<sub>3</sub> stock solution
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
 
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| align="center" style="background:#f0f0f0;"|''''''
 
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|-
 
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| Molecular Weight (g/mol)||101.1
 
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|-
 
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| mass(g)||4.044
 
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|-
 
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| volume stock (L)||0.05
 
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|-
 
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| Concentration (M)||0.8
 
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|}
 
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<br>
 
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Table 3. Preparation of Hemoglobin stock solution
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Preparation of hemoglobin Stock solution'''
 
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| align="center" style="background:#f0f0f0;"|''''''
 
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|-
 
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| Molecular weight (g/mol)||64500
 
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|-
 
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| mass hemglobin (g)||0.0995
 
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|-
 
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| volume solution (L)||0.1
 
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|-
 
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| Concentration (M)||1.54264E-05
 
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|}
 
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<br>
 
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Table 4. Preparation of KOH Stock solution
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
 
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| align="center" style="background:#f0f0f0;"|''''''
 
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|-
 
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| Molecular Weight (g/mol)||56.11
 
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|-
 
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| mass(g)||1.5
 
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|-
 
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| volume stock (L)||0.025
 
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|-
 
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| Concentration (M)||1.069328106
 
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|}
 
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<br>
 
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Table 5. Preparation of BSA stock solution
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock'''
 
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| align="center" style="background:#f0f0f0;"|''''''
 
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|-
 
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| Molecular weight (g/mol)||66463
 
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|-
 
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| mass (g)||0.0174
 
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|-
 
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| volume stock (L)||0.01
 
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|-
 
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| Concentration (M)||2.618E-05
 
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|}
 
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<br>
 
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Table 9. Preparation of Gold stock solution
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Preparation gold stock'''
 
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| align="center" style="background:#f0f0f0;"|''''''
 
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|-
 
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| Molecular weight Au (g/mol)||393.83
 
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|-
 
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| mass Au(g)||0.01446
 
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|-
 
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| volume stock(L)||0.01
 
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|-
 
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| Concentration (M)||0.003671635
 
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|}
 
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<br>
 
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Table 6. Preparation of Magnetite:BSA Reaction Mixtures
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
 
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| align="center" style="background:#f0f0f0;"|'''Concentration BSA (M)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume BSA Added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
 
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|-
 
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| 13000||2.30769E-06||0.440736074||0.5||0.467583333||2.5||1.091680592
 
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|-
 
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| 13500||2.22222E-06||0.424412516||0.5||0.467583333||2.5||1.108004151
 
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|}
 
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<br>
 
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Table 7. Preparation of Magnetite:Hemoglobin Reaction Mixture
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
 
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| align="center" style="background:#f0f0f0;"|'''Concentration Hemoglobin (M)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume Hemoglobin Added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
 
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|-
 
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| 16500||1.81818E-06||0.589310187||0.5||0.467583333||2.5||0.943106479
 
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|-
 
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| 17000||1.76471E-06||0.571977535||0.5||0.467583333||2.5||0.960439132
 
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|}
 
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<br>
 
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Table 8. Preparation of Au:BSA reaction mixtures
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
 
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| align="center" style="background:#f0f0f0;"|'''Amount of gold added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Concentration BSA (M)'''
 
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| align="center" style="background:#f0f0f0;"|'''volume BSA added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''volume water added (mL)'''
 
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|-
 
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| 130||0.340447787||1.92308E-06||0.367280062||4.292272151
 
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|-
 
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| 135||0.340447787||1.85185E-06||0.353677097||4.305875116
 
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|}
 
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<br>
 
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Table 9. Preparation of Au:Hemoglobin reaction mixtures
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
 
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| align="center" style="background:#f0f0f0;"|'''Amount of gold added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Concentration Hemoglobin (M)'''
 
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| align="center" style="background:#f0f0f0;"|'''volume Hemoglobin added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''volume water added (mL)'''
 
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|-
 
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| 165||0.340447787||1.51515E-06||0.491091823||4.16846039
 
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|-
 
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| 170||0.340447787||1.47059E-06||0.476647946||4.182904267
 
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|}
 
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<br>
 
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Revision as of 02:08, 18 April 2014

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