User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/19

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==Day 8==
 
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* Goals
 
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#Finish running UV-Vis on samples from yesterday
 
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# Make new Heme-Mag samples in petri dishes so that they may be analyzed using a microscope.
 
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# Run FTIR for samples which were dried [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/18|yesterday]]
 
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==Procedure==
 
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* For making the samples in the petri dish, we followed the sample protocol as we use for oven prep except this time we made 20mL of sample.
 
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Table 1. Preparation of FeSO<sub>4</sub>*7H<sub>2</sub>O stock solution
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
 
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| align="center" style="background:#f0f0f0;"|''''''
 
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|-
 
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| Molecular Weight (g/mol)||278.01
 
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|-
 
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| mass(g)||2.085075
 
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|-
 
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| volume stock (L)||0.025
 
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|-
 
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| Concentration (M)||0.3
 
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|}
 
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<br>
 
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Table 2. Preparation of KOH stock solution
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
 
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| align="center" style="background:#f0f0f0;"|''''''
 
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|-
 
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| Molecular Weight (g/mol)||56.11
 
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|-
 
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| mass(g)||1.5
 
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|-
 
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| volume stock (L)||0.025
 
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|-
 
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| Concentration (M)||1.069328106
 
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|}
 
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<br>
 
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Table 3. Preparation of KNO<sub>3</sub> stock solution
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
 
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| align="center" style="background:#f0f0f0;"|''''''
 
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|-
 
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| Molecular Weight (g/mol)||101.1
 
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|-
 
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| mass(g)||9.099
 
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|-
 
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| volume stock (L)||0.1
 
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|-
 
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| Concentration (M)||0.9
 
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|}
 
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<br>
 
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Table 4. Preparation of Hemoglobin stock solution
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Preparation of hemoglobin Stock solution'''
 
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| align="center" style="background:#f0f0f0;"|''''''
 
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|-
 
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| Molecular weight (g/mol)||64500
 
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|-
 
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| mass hemglobin (g)||0.0995
 
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|-
 
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| volume solution (L)||0.1
 
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|-
 
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| Concentration (M)||1.54264E-05
 
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|}
 
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<br>
 
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Table 5. Reaction Mixture preparation
 
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<br>
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
 
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| align="center" style="background:#f0f0f0;"|'''concentration Hemoglobin (M)'''
 
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| align="center" style="background:#f0f0f0;"|'''volume of Hemoglobin added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
 
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|-
 
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| 12500||0.0000024||3.111557789||2||1.870333333||8.888888889||4.129219989
 
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|-
 
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| 13000||2.30769E-06||2.991882489||2||1.870333333||8.888888889||4.248895288
 
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|-
 
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| 13500||2.22222E-06||2.881072027||2||1.870333333||8.888888889||4.359705751
 
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|-
 
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| 14000||2.14286E-06||2.778176597||2||1.870333333||8.888888889||4.462601181
 
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|-
 
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| 14500||2.06897E-06||2.682377404||2||1.870333333||8.888888889||4.558400374
 
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|-
 
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| 15000||0.000002||2.592964824||2||1.870333333||8.888888889||4.647812954
 
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|-
 
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| 15500||1.93548E-06||2.509320798||2||1.870333333||8.888888889||4.73145698
 
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|-
 
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| 16000||0.000001875||2.430904523||2||1.870333333||8.888888889||4.809873255
 
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|-
 
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| 16500||1.81818E-06||2.357240749||2||1.870333333||8.888888889||4.883537029
 
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|-
 
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| 17000||1.76471E-06||2.287910139||2||1.870333333||8.888888889||4.952867639
 
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|}
 
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<br>
 
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*These samples will be analyzed using a microscope next lab.
 
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<br>
 
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==Data==
 
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*For FTIR and UV-Vis spectras collected today, please see [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/19|Moira's notebook]]
 
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Revision as of 01:11, 18 April 2014

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