User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/12

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Current revision (22:49, 19 April 2014) (view source)
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Day 6==
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* Goals
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**Prepare hemoglobin-magnetite solutions at the new ratios in the oven for comparison to the ones made of BSA. The BSA- Magnetite reaction mixtures at these ratios appeared to have more fiber like precipitates.
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**UV-Vis the samples prepared on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11|Yesterday]]
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** Separate out the magnetite nanoparticles/ fibers using a magnet from the solutions prepared [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11|Yesterday]] for future use in FTIR and XRD analysis
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**Analyze the magnetite nanoparticles/ fibers separated [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11| Yesterday]] using  FTIR.
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==Procedure==
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* We prepared the stock solutions of FeSO<sub>4</sub>*7H<sub>2</sub>O ,KNO<sub>3</sub>, and KOH in the same method and at the same concentrations as prepared on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04|February 4]]. The same hemoglobin stock solution as made [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/11| yesterday]] was used.
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* We made the following ten ratios for the oven method. the same procedure as used on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04|1/29]]
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Table 1: Preparation of Reaction Mixtures for Hemeglobin- Magnetite Nanoparticle/Fiber formation
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''concentration Hemoglobin (M)'''
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| align="center" style="background:#f0f0f0;"|'''volume of Hemoglobin added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
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| 10500||2.85714E-06||0.926058866||0.5||0.500624554||2.5||0.57331658
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| 11000||2.72727E-06||0.883965281||0.5||0.500624554||2.5||0.615410165
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| 11500||2.6087E-06||0.845532008||0.5||0.500624554||2.5||0.653843438
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| 12000||0.0000025||0.810301508||0.5||0.500624554||2.5||0.689073939
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| 12500||0.0000024||0.777889447||0.5||0.500624554||2.5||0.721485999
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| 13000||2.30769E-06||0.747970622||0.5||0.500624554||2.5||0.751404824
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| 13500||2.22222E-06||0.720268007||0.5||0.500624554||2.5||0.779107439
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| 14000||2.14286E-06||0.694544149||0.5||0.500624554||2.5||0.804831297
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| 14500||2.06897E-06||0.670594351||0.5||0.500624554||2.5||0.828781095
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| 15000||0.000002||0.648241206||0.5||0.500624554||2.5||0.85113424
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|}
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<br>
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==Data==
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* For FTIR spectras and peaks, please see [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/12| Moira's Notebook]]
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|}
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==Observations==
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* For the oven prep reaction mixtures. It was noticed that immediately after adding the  FeSO<sub>4</sub>*7H<sub>2</sub>O a dark(dark green/blue) precipitate in both small clusters and long string like shape formed in the Heme:Magnetite samples. We always add FeSO<sub>4</sub>*7H<sub>2</sub>O last into the solutions so that it does not prematurely react with the other compounds and to make sure it does not inhibit the reaction from proceeding.
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*Upon observing the BSA:Magnetite samples, it appeared that large clusters formed (leaf like structures) at the 12500:1 ratio and continued to form up to the 15000:1 ratio. Prior to the 12500:1 ratio, it appears a magnetic colloid suspension was formed, indicating that all ratios successfully formed magnetite nanoparticles. However, for potential fiber formation, it appears the 12500:1-15000:1 range has potential.
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__NOTOC__
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