User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/05

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(Procedure)
Current revision (22:48, 19 April 2014) (view source)
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
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==Day 4==
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* GOALS:
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#Prepare  BSA with magnetite using the general protocol described on 1/28 and 1/29 for comparison. (oven and nitrogen)
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#UV-Vis of samples prepared on 1/29 and 2/4 (Magnetite-BSA made under nitrogen and Magnetite-BSA in oven and Hemoglobin+magnetite made in oven and under nitrogen.
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#UV Vis protocol described on 1/29 in [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/29|Moira’s Open wet ware]]
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#Prepare all the magnetite samples from last week for FTIR and X-ray analysis. 
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==Procedure==
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'''Preparation of BSA-Magnetitie nanoparticles'''
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* We followed our general procedure from [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29|1/29]].
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* We prepared two different solutions, one with the correct KNO<sub>3</sub> concentration and one with the incorrect one. For details on incorrect and correct concentrations see [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04|2/4]]
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Table 1. Preparation of both reaction mixtures
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''concentration BSA (M)'''
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| align="center" style="background:#f0f0f0;"|'''volume of BSA added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''volume water added (mL)'''
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|-
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| 13000||2.30769E-06||0.459210041||0.5||0.500624554||2.5||1.040165405
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|}
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<br>
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Table 2. Preparation of BSA stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular weight (g/mol)||66463
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|-
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| mass (g)||0.0167
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|-
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| volume stock (L)||0.01
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| Concentration (M)||2.51268E-05
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|}
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<br>
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Table 3. Preparation of FeSO<sub>4</sub>*7H<sub>2</sub>O stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular Weight (g/mol)||278.01
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|-
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| mass(g)||0.83403
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|-
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| volume stock (L)||0.01
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|-
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| Concentration (M)||0.3
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|}
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<br>
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Table 4. Preparation of KOH stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular Weight (g/mol)||56.11
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|-
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| mass(g)||0.5604
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|-
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| volume stock (L)||0.01
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| Concentration (M)||0.998752451
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<br>
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Table 5. Preparation of KNO<sub>3</sub> stock solution for Reaction Mixture 1
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular Weight (g/mol)||101.1
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|-
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| mass(g)||4.044
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|-
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| volume stock (L)||0.05
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| Concentration (M)||0.8
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<br>
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Table 6. Preparation of KNO<sub>3</sub> stock solution for Reaction Mixture 2
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular Weight (g/mol)||101.1
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|-
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| mass(g)||0.5604
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|-
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| volume stock (L)||0.01
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|-
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| Concentration (M)||0.5
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|}
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'''FTIR and XRD sample Prep'''
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For details on this process and procedure please see [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/05|Moira's notebook]]
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==DATA==
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*All samples prepared on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04|2/4]] were analyzed using UV/vis spectroscopy.
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*The samples were not centrifuged because the nanoparticles/fibers were removed from the solution in preparation for FTIR and XRD analysis prior to UV/vis analysis.
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<br>
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'''Figure 1.''' Corrected Absorbance Spectra of Hemoglobin:Magnetite nanoparticles/fibers
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<br>
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[[Image:Corrected Absorbance Spectra of Heme-Magnetite nanoparticles .png]]
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<br>
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*Strong absorbance peak around 302nm corresponds to the excess nitrate present from the magnetite synthesis. The weak absorbance peaks
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'''Figure 2.''' Corrected Absorbance Spectra of BSA:Magnetite nanoparticles/fibers
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<br>
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[[Image:Corrected Absorbance Spectra of BSA-Magnetite nanoparticles .png]]
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==Observations & Notes==
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*We were unable to run AAS as we do not have an Iron Lamp for the AA at this time. The reaction mixtures (minus the precipitants) have been stored for now.
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* Please see [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/05|Moira's Notebook]] for observations on particle size and magnetic characteristics observed.
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<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
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|}
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__NOTOC__
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