User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04

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(Procedure)
Current revision (21:48, 19 April 2014) (view source)
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
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==Day 3==
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* Goals:
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# Prepare a Au:Hemoglobin solution using the method of protein- AuNP preparation found [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/28|Here]] (It is the same as the Au-BSA solutions prepared). These samples will be used for comparison against the new method preparation done today. 
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#Prepare two Magnetitic and hemoglobin solutions. The first  following the protocol described on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29|Wednesday]]. Everything will once again be constantly de-gassed with nitrogen using the Schlenk line. And the second following the same ratio and protocol listed on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/28|Tuesday]]. The preparation of these two samples will allow for comparison in the future.
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#Filter the Magnetite:Au-BSA solutions from [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29|Last week]] using a Whatman filter paper with a pore size of 20-25μ. This filtrate will be dried and analyzed using DSC, FTIR and XRD.
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#Potentiall place some of the prepared fibers in water in order to test their stability. 
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#Test magnetic property of the fibers using a magnet/magnetometer.
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#Centrifuge recollected solution for future AA analysis.
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==Procedure==
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'''Hemeoglobin+ Mangetite Solution made using a Schlenk Line'''
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The same procedure as described on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29|1/29]] was used.
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*Preparation of the Stock solutions: After starting the synthesis reaction, we discovered that the  the synthesis, it was discovered that the KNO<sub>3</sub> stock was prepared incorrectly, resulting in the concentration listed on table 4. The concentration was supposed to be 0.8M. A new synthesis of this specific reaction and method will need to be performed with the correct concentrations.
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Table 1. Preparation of Hemoglobin stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of hemoglobin Stock solution'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular weight (g/mol)||64500
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|-
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| mass hemglobin (g)||0.1
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| volume solution (L)||0.1
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| Concentration (M)||1.55039E-05
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|}
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<br>
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Table 2. Preparation of FeSO<sub>4</sub>*7H<sub>2</sub>O stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular Weight (g/mol)||278.01
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|-
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| mass(g)||0.83403
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| volume stock (L)||0.01
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| Concentration (M)||0.3
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|}
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<br>
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Table 3. Preparation of KOH stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular Weight (g/mol)||56.11
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| mass(g)||0.5606
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| volume stock (L)||0.01
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| Concentration (M)||0.999108893
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|}
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<br>
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Table 4. Preparation of KNO<sub>3</sub> stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular Weight (g/mol)||101.1
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| mass(g)||0.5604
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| volume stock (L)||0.01
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| Concentration (M)||0.5
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Table 5. Preparation of Hemoglobin:Magnetite solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''concentration Hemoglobin (M)'''
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| align="center" style="background:#f0f0f0;"|'''volume of Hemoglobin added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
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|-
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| 13000||2.30769E-06||0.744230769||0.5||0.500445951||2.5||0.75532328
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|}
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<br>
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*The ratio is two orders of magnitude larger than the ratio when gold is used for prep due to the fact this ratio refers to the concentration of FeSO<sub>4</sub> in solution (which is 0.03M). As such, the ratio was altered to make similar concentration comparisons (with this order of magnitude it is comparing to 0.003M which is similar to the 0.0025M gold in the previous solutions)
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<br>
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'''The second method of preparation used was via oven preparation.'''
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The same procedure as described on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29|1/29] was used. However, gold was not added to the reaction mixture (these steps were not included).
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<br>
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Table 1. Preparation of KNO<sub>3</sub> stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular Weight (g/mol)||101.1
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| mass(g)||4.044
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| volume stock (L)||0.05
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| Concentration (M)||0.8
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<br>
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Table 2. Preparation of KOH stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular Weight (g/mol)||56.11
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|-
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| mass(g)||0.5604
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|-
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| volume stock (L)||0.01
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| Concentration (M)||0.998752451
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|}
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<br>
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Table 3. Preparation of Hemoglobin:Magnetite solution using the oven
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''concentration Hemoglobin (M)'''
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| align="center" style="background:#f0f0f0;"|'''volume of Hemoglobin added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
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|-
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| 10000||0.000003||0.9675||0.5||0.500624554||2.5||0.531875446
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|-
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| 13000||2.30769E-06||0.744230769||0.5||0.500624554||2.5||0.755144677
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|}
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<br>
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*The FeSO<sub>4</sub>*7H<sub>2</sub>O stock solution described in Table 2 was used for this synthesis
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<br>
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*We also centrifuged the Hemoglobin- magnetite- gold samples and made 4 new ratios of hemoglobin and magnetite
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<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
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|}
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==PHOTO DATA==
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[[Image:BSA mag 2-4.jpeg|650px]]
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_NOTOC__
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