User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04

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'''The second method of preparation used was via oven preparation.'''
'''The second method of preparation used was via oven preparation.'''
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The same procedure as described on [User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29|1/29] was used. |1/28]] was followed. However, gold was not added to the reaction mixture (these steps were not included).
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The same procedure as described on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29|1/29] was used. |1/28]] was followed. However, gold was not added to the reaction mixture (these steps were not included).
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Table 1. Preparation of KNO<sub>3</sub> stock solution
Table 1. Preparation of KNO<sub>3</sub> stock solution

Revision as of 14:00, 24 February 2014

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Day 3

  • Goals:
  1. Prepare a Au:Hemoglobin solution using the method of protein- AuNP preparation found Here (It is the same as the Au-BSA solutions prepared). These samples will be used for comparison against the new method preparation done today.
  2. Prepare two Magnetitic and hemoglobin solutions. The first following the protocol described on Wednesday. Everything will once again be constantly de-gassed with nitrogen using the Schlenk line. And the second following the same ratio and protocol listed on Tuesday. The preparation of these two samples will allow for comparison in the future.
  3. Filter the Magnetite:Au-BSA solutions from Last week using a Whatman filter paper with a pore size of 20-25μ. This filtrate will be dried and analyzed using DSC, FTIR and XRD.
  4. Potentiall place some of the prepared fibers in water in order to test their stability.
  5. Test magnetic property of the fibers using a magnet/magnetometer.
  6. Centrifuge recollected solution for future AA analysis.

Procedure

Hemeoglobin+ Mangetite Solution made using a Schlenk Line The same procedure as described on 1/29 was used.

  • Preparation of the Stock solutions: After starting the synthesis reaction, we discovered that the the synthesis, it was discovered that the KNO3 stock was prepared incorrectly, resulting in the concentration listed on table 4. The concentration was supposed to be 0.8M. A new synthesis of this specific reaction and method will need to be performed with the correct concentrations.

Table 1. Preparation of Hemoglobin stock solution

Preparation of hemoglobin Stock solution '
Molecular weight (g/mol)64500
mass hemglobin (g)0.1
volume solution (L)0.1
Concentration (M)1.55039E-05


Table 2. Preparation of FeSO4*7H2O stock solution

Preparation of FeSO4 stock '
Molecular Weight (g/mol)278.01
mass(g)0.83403
volume stock (L)0.01
Concentration (M)0.3


Table 3. Preparation of KOH stock solution

Preparation of KOH stock '
Molecular Weight (g/mol)56.11
mass(g)0.5606
volume stock (L)0.01
Concentration (M)0.999108893


Table 4. Preparation of KNO3 stock solution

Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)0.5604
volume stock (L)0.01
Concentration (M)0.5

Table 5. Preparation of Hemoglobin:Magnetite solution

Ratio concentration Hemoglobin (M) volume of Hemoglobin added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
130002.30769E-060.7442307690.50.5004459512.50.75532328


  • The ratio is two orders of magnitude larger than the ratio when gold is used for prep due to the fact this ratio refers to the concentration of FeSO4 in solution (which is 0.03M). As such, the ratio was altered to make similar concentration comparisons (with this order of magnitude it is comparing to 0.003M which is similar to the 0.0025M gold in the previous solutions)


The second method of preparation used was via oven preparation. The same procedure as described on 1/29] was used. |1/28 was followed. However, gold was not added to the reaction mixture (these steps were not included).
Table 1. Preparation of KNO3 stock solution

Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)4.044
volume stock (L)0.05
Concentration (M)0.8


Table 2. Preparation of KOH stock solution

Preparation of KOH stock '
Molecular Weight (g/mol)56.11
mass(g)0.5604
volume stock (L)0.01
Concentration (M)0.998752451


Table 3. Preparation of Hemoglobin:Magnetite solution using the oven

Ratio concentration Hemoglobin (M) volume of Hemoglobin added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
100000.0000030.96750.50.5006245542.50.531875446
130002.30769E-060.7442307690.50.5006245542.50.755144677


  • The FeSO4*7H2O stock solution described in Table 2 was used for this synthesis


  • We also centrifuged the Hemoglobin- magnetite- gold samples and made 4 new ratios of hemoglobin and magnetite


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