User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/28

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(Procedure)
Current revision (11:15, 18 April 2014) (view source)
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{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
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==Day 1==
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*Goals:
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# Prepare 3 Bovine Serum Albumin (BSA) and gold nanoparticle protein fibers as a standard for comparison against the solutions containing BSA, Gold, and magnetite.
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#Determine the ideal concentration necessary to prepare BSA protein fibers with gold and magnetite nanoparticles by preparing 10 solutions of varying concentration ratios. 
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==Procedure==
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*Preparation protocol for AuNPs in BSA solution.
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#Prepare separate stock solution of each BSA and HAuCl<sub>4</sub> in volumetric flasks deionized water.
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#Add the necessary volumes of gold and BSA to achieve the desired concentration ratios (BSA:AU) into 10 test tubes. The total volume of the samples should not exceed 5mL.
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#Add the necessary amount of deionized H<sub>2</sub>O  into the test tubes in order to achieve a final volume in each test tube of 5mL
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#Cap the test tubes with an aluminum cap and heat in an oven for 3 hours at 80°C.
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#Store the remaining solutions in falcon tubes.
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*Preparation of Magnetite-AuNP-BSA Solutions
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**These solutions were prepared following the same method as the BSA-AuNP solutions.
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**The following concentrations of FeSO<sub>4</sub>*7H<sub>2</sub>O, KOH, and KNO<sub>3</sub> in each prepared solution were adapted from the following reference[[http://www.sciencedirect.com/science/article/pii/S002197970901488X]]
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Protocol:
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#Prepare the following two stock solutions in a 10mL volumetric flask using deionized H<sub>2</sub>O. Prepare KNO<sub>3</sub> in a 50mL volumetric flask.
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##0.3M solution of  FeSO<sub>4</sub>*7H<sub>2</sub>O
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##1M  solution of KOH i
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##0.8M solution of KNO<sub>3</sub>.
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#Add the appropriate volume of BSA and gold in a 10mL test tube to obtain the desired ratio of BSA:Au. Adjust levels so that the total volume of the solution does not exceed 5mL at this time. 
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#Add the appropriate volume of FeSO<sub>4</sub>*7H<sub>2</sub>O to each test tube so that the solution has a concentration of 0.03M FeSO<sub>4</sub>*7H<sub>2</sub>O.
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#Add the appropriate volume of KOH to each test tube so that the solution has a concentration of 0.1M KOH.
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#Add the appropriate volume of KNO<sub>3</sub> to each test tube so that the solution has a concentration of 0.4M KNO<sub>3</sub>.
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#Add deionized water to all of the test tube samples in order to bring up all the solutions to 5mL. Cap the test tubes with aluminum caps and heat them in the oven for 3 hours at 80°C.
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#Store any remaining stock solutions in falcon tubes.
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'''Note''':Use caution when removing the test tube from oven. The test tubes will be hot.
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==Calculations==
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*Preparation of BSA and Gold.
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Table 1. Preparation of BSA Stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular weight (g/mol)||66463
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|-
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| mass (g)||0.0164
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| volume stock (L)||0.01
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| Concentration (M)||2.46754E-05
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|}
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<br>
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Table 2. Preparation of Gold Stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation gold stock'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular weight Au (g/mol)||393.83
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| mass Au(g)||0.0326
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| volume stock(L)||0.025
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| Concentration (M)||0.003311073
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|}
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<br>
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Table 3. Preparation of BSA-AuNP solutions- We chose these three ratios based on the knowledge of the fiber producing region from previous experiement.s
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Amount of gold added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume BSA added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume water added (mL)'''
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|-
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| 125||0.377521089||0.405262195||4.217216716
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|-
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| 130||0.377521089||0.389675188||4.232803723
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|-
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| 135||0.377521089||0.375242773||4.247236138
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|}
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<br>
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* Preparation of Magnetite, AuNPs, and BSA
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Table 4. Preparation of FeSO<sub>4</sub>*7H<sub>2</sub>O stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular Weight (g/mol)||278.01
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|-
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| mass(g)||0.83403
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|-
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| volume stock (L)||0.01
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| Concentration (M)||0.3
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|}
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<br>
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Table 5. Preparation of the KOH stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular Weight (g/mol)||56.11
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| mass(g)||0.5606
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| volume stock (L)||0.01
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| Concentration (M)||0.999108893
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<br>
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Table 6. Preparation of KNO<sub>3</sub> stock solution
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Molecular Weight (g/mol)||101.1
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| mass(g)||4.044
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| volume stock (L)||0.05
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| Concentration (M)||0.8
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|}
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<br>
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Table 7. Preparation of the Magnetite nanoparticle:AuNP:BSA solutions. Because we did not know where to expect fibers to form. We chose a wide range of ratios to test.
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Amount of gold added(mL)'''
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| align="center" style="background:#f0f0f0;"|'''concentration BSA (M)'''
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| align="center" style="background:#f0f0f0;"|'''volume BSA (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
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|-
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| 105||0.377521089||2.38095E-06||0.482454994||0.5||0.500445951||2.5||0.639577966
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| 110||0.377521089||2.27273E-06||0.460525222||0.5||0.500445951||2.5||0.661507739
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| 115||0.377521089||2.17391E-06||0.440502386||0.5||0.500445951||2.5||0.681530574
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| 120||0.377521089||2.08333E-06||0.42214812||0.5||0.500445951||2.5||0.69988484
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| 125||0.377521089||0.000002||0.405262195||0.5||0.500445951||2.5||0.716770765
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| 130||0.377521089||1.92308E-06||0.389675188||0.5||0.500445951||2.5||0.732357773
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| 135||0.377521089||1.85185E-06||0.375242773||0.5||0.500445951||2.5||0.746790187
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| 140||0.377521089||1.78571E-06||0.361841246||0.5||0.500445951||2.5||0.760191715
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| 145||0.377521089||1.72414E-06||0.349363961||0.5||0.500445951||2.5||0.772668999
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| 150||0.377521089||1.66667E-06||0.337718496||0.5||0.500445951||2.5||0.784314464
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<br>
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==Observations==
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*The addition of the FeSO<sub>4</sub>*7H<sub>2</sub>O  solution to the gold caused an immediate formation of the gold nanoparticles through the reduction of the free gold by the  FeSO<sub>4</sub>*7H<sub>2</sub>O. This reaction was depicted by an immediate change in the color of the sample solutions from a yellowish hue (characteristic of the gold solution) to a deep/ dark purple. 
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<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
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|}
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__NOTOC__
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