User:Pranav Rathi/Notebook/OT/2012/09/21/Fiber light for Olympus microscope - Revision history

Pranav Rathi: /* Demonstration */

Pranav Rathi — Sat, 26 Jan 2013 16:49:06 GMT

Demonstration

←Older revision		Revision as of 16:49, 26 January 2013
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	== Demonstration ==		== Demonstration ==
-	I am using fiber-light for months now for all my experiments the intensity field looks uniform and constant. Below is the video I recorded: DNA-tethering in D2O sample. The ~~hike~~ in the intensity in the video are due to recording and video processing.	+	I am using fiber-light for months now for all my experiments the intensity field looks uniform and constant. Below is the video I recorded: DNA-tethering in D2O sample. The flicks in the intensity in the video are due to recording and video processing not due to fiber light.

	{{#widget:YouTube\|id=ihl45JssNTA}}		{{#widget:YouTube\|id=ihl45JssNTA}}

Pranav Rathi: /* Demonstration */

Pranav Rathi — Sat, 26 Jan 2013 16:48:22 GMT

Demonstration

←Older revision		Revision as of 16:48, 26 January 2013
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	== Demonstration ==		== Demonstration ==
-	I an using fiber-light for months now. Below is the video I recorded: DNA-~~ethering~~ in D2O sample.	+	I am using fiber-light for months now for all my experiments the intensity field looks uniform and constant. Below is the video I recorded: DNA-tethering in D2O sample. The hike in the intensity in the video are due to recording and video processing.
		+
	{{#widget:YouTube\|id=ihl45JssNTA}}		{{#widget:YouTube\|id=ihl45JssNTA}}

Pranav Rathi at 23:46, 25 January 2013

Pranav Rathi — Fri, 25 Jan 2013 23:46:13 GMT

←Older revision		Revision as of 23:46, 25 January 2013
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		+	==Introduction==
		+	I have invented a fiber-light system for Olympus microscope IX71. This system is compatible to Olympus power supply TH4-100. The system has 4 parts: Olympus power supply, Dolan Jenner fiber bundle, fiber-feeder and illuminator & fiber plugin adapter. Fiber plugin adapter can be directly mounted on the illuminator and the whole system can be controlled through Olympus power supply. I designed it such that it requires least amount of machining with regular tools and components available in any optics lab.

	<div align="center"><html><iframe src="http://www.slideshare.net/slideshow/embed_code/16182938" width="600" height="400" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" style="border:1px solid #CCC;border-width:1px 1px 0;margin-bottom:5px" allowfullscreen webkitallowfullscreen mozallowfullscreen> </iframe> <div style="margin-bottom:5px"> <strong> <a href="http://www.slideshare.net/pranavrathi/fiber-light-for-olympus-microscope" title="Fiber light for olympus microscope " target="_blank">Fiber light for olympus microscope </a> </strong> from <strong><a href="http://www.slideshare.net/pranavrathi" target="_blank">pranavrathi</a></strong> </div></html></div>		<div align="center"><html><iframe src="http://www.slideshare.net/slideshow/embed_code/16182938" width="600" height="400" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" style="border:1px solid #CCC;border-width:1px 1px 0;margin-bottom:5px" allowfullscreen webkitallowfullscreen mozallowfullscreen> </iframe> <div style="margin-bottom:5px"> <strong> <a href="http://www.slideshare.net/pranavrathi/fiber-light-for-olympus-microscope" title="Fiber light for olympus microscope " target="_blank">Fiber light for olympus microscope </a> </strong> from <strong><a href="http://www.slideshare.net/pranavrathi" target="_blank">pranavrathi</a></strong> </div></html></div>

-
-
-
-
-
-	~~==Introduction==~~
-	I have invented a fiber-light system for Olympus microscope IX71. This system is compatible to Olympus power supply TH4-100. The system has 4 parts: Olympus power supply, Dolan Jenner fiber bundle, fiber-feeder and illuminator & fiber plugin adapter. Fiber plugin adapter can be directly mounted on the illuminator and the whole system can be controlled through Olympus power supply. I designed it such that it requires least amount of machining with regular tools and components available in any optics lab.

	==Motivation==		==Motivation==

Pranav Rathi: /* Introduction */

Pranav Rathi — Fri, 25 Jan 2013 23:45:11 GMT

Introduction

←Older revision	Revision as of 23:45, 25 January 2013
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	+
	+
	+	<div align="center"><html><iframe src="http://www.slideshare.net/slideshow/embed_code/16182938" width="600" height="400" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" style="border:1px solid #CCC;border-width:1px 1px 0;margin-bottom:5px" allowfullscreen webkitallowfullscreen mozallowfullscreen> </iframe> <div style="margin-bottom:5px"> <strong> <a href="http://www.slideshare.net/pranavrathi/fiber-light-for-olympus-microscope" title="Fiber light for olympus microscope " target="_blank">Fiber light for olympus microscope </a> </strong> from <strong><a href="http://www.slideshare.net/pranavrathi" target="_blank">pranavrathi</a></strong> </div></html></div>
	+
	+
	+
	+

Pranav Rathi at 23:44, 25 January 2013

Pranav Rathi — Fri, 25 Jan 2013 23:44:32 GMT

←Older revision		Revision as of 23:44, 25 January 2013
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-	<div align="center"><html><iframe src="http://www.slideshare.net/slideshow/embed_code/16182938" width="427" height="356" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" style="border:1px solid #CCC;border-width:1px 1px 0;margin-bottom:5px" allowfullscreen webkitallowfullscreen mozallowfullscreen> </iframe> <div style="margin-bottom:5px"> <strong> <a href="http://www.slideshare.net/pranavrathi/fiber-light-for-olympus-microscope" title="Fiber light for olympus microscope " target="_blank">Fiber light for olympus microscope </a> </strong> from <strong><a href="http://www.slideshare.net/pranavrathi" target="_blank">pranavrathi</a></strong> </div></html></div>	+

	==Introduction==		==Introduction==

Pranav Rathi at 23:43, 25 January 2013

Pranav Rathi — Fri, 25 Jan 2013 23:43:56 GMT

←Older revision		Revision as of 23:43, 25 January 2013
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		+	<div align="center"><html><iframe src="http://www.slideshare.net/slideshow/embed_code/16182938" width="427" height="356" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" style="border:1px solid #CCC;border-width:1px 1px 0;margin-bottom:5px" allowfullscreen webkitallowfullscreen mozallowfullscreen> </iframe> <div style="margin-bottom:5px"> <strong> <a href="http://www.slideshare.net/pranavrathi/fiber-light-for-olympus-microscope" title="Fiber light for olympus microscope " target="_blank">Fiber light for olympus microscope </a> </strong> from <strong><a href="http://www.slideshare.net/pranavrathi" target="_blank">pranavrathi</a></strong> </div></html></div>
		+
	==Introduction==		==Introduction==
	I have invented a fiber-light system for Olympus microscope IX71. This system is compatible to Olympus power supply TH4-100. The system has 4 parts: Olympus power supply, Dolan Jenner fiber bundle, fiber-feeder and illuminator & fiber plugin adapter. Fiber plugin adapter can be directly mounted on the illuminator and the whole system can be controlled through Olympus power supply. I designed it such that it requires least amount of machining with regular tools and components available in any optics lab.		I have invented a fiber-light system for Olympus microscope IX71. This system is compatible to Olympus power supply TH4-100. The system has 4 parts: Olympus power supply, Dolan Jenner fiber bundle, fiber-feeder and illuminator & fiber plugin adapter. Fiber plugin adapter can be directly mounted on the illuminator and the whole system can be controlled through Olympus power supply. I designed it such that it requires least amount of machining with regular tools and components available in any optics lab.

Pranav Rathi: /* Alignment */

Pranav Rathi — Fri, 25 Jan 2013 23:36:30 GMT

Alignment

←Older revision		Revision as of 23:36, 25 January 2013
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	==Alignment==		==Alignment==
	Do not touch the box when the lamp is on for a while it can be very hot. To optimize the light through the fiber do following:		Do not touch the box when the lamp is on for a while it can be very hot. To optimize the light through the fiber do following:
-	* Like in slide 27 put the fiber through the fiber outlet, turn on the lamp and keep it low (avoid looking ~~direct~~ into the lamp).	+	* Like in slide 27 put the fiber through the fiber outlet, turn on the lamp and keep it low (avoid looking directly into the lamp).
	* Adjust the cage plates for focus and adjust the 90 deg clamp for xy adjustment of the focal spot over fiber opening; slide 28. Once the spot is on the fiber like in slide 29 you can further adjust the fiber outlet cage plate.		* Adjust the cage plates for focus and adjust the 90 deg clamp for xy adjustment of the focal spot over fiber opening; slide 28. Once the spot is on the fiber like in slide 29 you can further adjust the fiber outlet cage plate.
	* To bring the fiber in focus on illuminator adjust the cage plate setting on the fiber plugin adapter like in slide 18 and 30 ( DO NOT look into the illuminator if it is too bright). Keep adjusting until you see the fiber crispy and big.		* To bring the fiber in focus on illuminator adjust the cage plate setting on the fiber plugin adapter like in slide 18 and 30 ( DO NOT look into the illuminator if it is too bright). Keep adjusting until you see the fiber crispy and big.

Pranav Rathi: /* Lamp Mount */

Pranav Rathi — Fri, 25 Jan 2013 23:33:55 GMT

Lamp Mount

←Older revision		Revision as of 23:33, 25 January 2013
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	=====Lamp Mount=====		=====Lamp Mount=====
-	This is the trickiest and most important part. This part depends on what materials you use, if you ceramic besides PCB then the procedure would be little different. I used PCB because the ceramic was not available in my lab. Get a plane PCB plates cut them in size of 1.5X2 inches get 6 of them and stack them together; slide 21 and 22. Make 3/8’’ through holes in all the plates on long side to align the holes made on the box-wall on lamp side vertically. Prepare two peace of 1/4 inch thick metal (choose you own dimensions) as shown in the slide 22. Make 2 3/8 inche holes on each side of each plate. Drill one through hole on each plate to fit the lamp connector pins (this is important to be very careful). Take 3 PCB plates and make 4 4/40 inch through holes to align the 4 holes on the 2 metal plates. Take 2 PCB plates and make 4 4/40 ~~screw’s~~ head size holes to fit the 4/40 screw heads. Leave the left over PCB plate alone. Now stack them in this order: put 4/40 screws in 3 (4/40 inch size hole) PCB plates in front, 2 (4/40 inch screw‘s head size) plate after that and the leftover PCB plate in the end. Now put the metal plates on the 4 screws and tight the nuts. Since the holes on the metal plates are bigger than screws you can use washers now. Mount the PCB plates on the vertical holes on the box. Mount the lamp on the metal plates, to fit the lamp connectors you can now slide the plates in and out because the plate holes are lot bigger than the screws, once find the right setting (lamp should not be loose) tight the nuts and solder the banana connectors to the plates. Check the lamp if it works.	+	This is the trickiest and most important part. This part depends on what materials you use, if you ceramic besides PCB then the procedure would be little different. I used PCB because the ceramic was not available in my lab. Get a plane PCB plates cut them in size of 1.5X2 inches get 6 of them and stack them together; slide 21 and 22. Make 3/8’’ through holes in all the plates on long side to align the holes made on the box-wall on lamp side vertically. Prepare two peace of 1/4 inch thick metal (choose you own dimensions) as shown in the slide 22. Make 2 3/8 inche holes on each side of each plate. Drill one through hole on each plate to fit the lamp connector pins (this is important to be very careful). Take 3 PCB plates and make 4 4/40 inch through holes to align the 4 holes on the 2 metal plates. Take 2 PCB plates and make 4 4/40 screw-head size holes to fit the 4/40 screw heads. Leave the left over PCB plate alone. Now stack them in this order: put 4/40 screws in 3 (4/40 inch size hole) PCB plates in front, 2 (4/40 inch screw‘s head size) plate after that and the leftover PCB plate in the end. Now put the metal plates on the 4 screws and tight the nuts. Since the holes on the metal plates are bigger than screws you can use washers now. Mount the PCB plates on the vertical holes on the box. Mount the lamp on the metal plates, to fit the lamp connectors you can now slide the plates in and out because the plate holes are lot bigger than the screws, once find the right setting (lamp should not be loose) tight the nuts and solder the banana connectors to the plates. Check the lamp if it works.

	=====Fiber outlet adapter=====		=====Fiber outlet adapter=====

Pranav Rathi: /* Lamp Mount */

Pranav Rathi — Fri, 25 Jan 2013 23:32:43 GMT

Lamp Mount

←Older revision		Revision as of 23:32, 25 January 2013
Line 101:		Line 101:

	=====Lamp Mount=====		=====Lamp Mount=====
-	This is the trickiest and most important part. This part depends on what materials you use, if you ceramic besides PCB then the procedure would be little different. I used PCB because the ceramic was not available in my lab. Get a plane PCB plates cut them in size of 1.5X2 inches get 6 of them and stack them together; slide 21 and 22. Make 3/8’’ through holes in all the plates on long side to align the holes made on the box-wall on lamp side vertically. Prepare two peace of 1/4 inch thick metal (choose you own dimensions) as shown in the slide 22. Make 2 3/8'' holes on each side of each plate. Drill one through hole on each plate to fit the lamp connector pins (this is important to be very careful). Take 3 PCB plates and make 4 4/~~40’’~~ through holes to align the 4 holes on the 2 metal plates. Take 2 PCB plates and make 4 4/40 screw’s head size holes to fit the 4/40 screw heads. Leave the left over PCB plate alone. Now stack them in this order: put 4/40 screws in 3 (4/~~40’’~~ size hole) PCB plates in front, 2 (4/~~40’’~~ screw‘s head size) plate after that and the leftover PCB plate in the end. Now put the metal plates on the 4 screws and tight the nuts. Since the holes on the metal plates are bigger than screws you can use washers now. Mount the PCB plates on the vertical holes on the box. Mount the lamp on the metal plates, to fit the lamp connectors you can now slide the plates in and out because the plate holes are lot bigger than the screws, once find the right setting (lamp should not be loose) tight the nuts and solder the banana connectors to the plates. Check the lamp if it works.	+	This is the trickiest and most important part. This part depends on what materials you use, if you ceramic besides PCB then the procedure would be little different. I used PCB because the ceramic was not available in my lab. Get a plane PCB plates cut them in size of 1.5X2 inches get 6 of them and stack them together; slide 21 and 22. Make 3/8’’ through holes in all the plates on long side to align the holes made on the box-wall on lamp side vertically. Prepare two peace of 1/4 inch thick metal (choose you own dimensions) as shown in the slide 22. Make 2 3/8 inche holes on each side of each plate. Drill one through hole on each plate to fit the lamp connector pins (this is important to be very careful). Take 3 PCB plates and make 4 4/40 inch through holes to align the 4 holes on the 2 metal plates. Take 2 PCB plates and make 4 4/40 screw’s head size holes to fit the 4/40 screw heads. Leave the left over PCB plate alone. Now stack them in this order: put 4/40 screws in 3 (4/40 inch size hole) PCB plates in front, 2 (4/40 inch screw‘s head size) plate after that and the leftover PCB plate in the end. Now put the metal plates on the 4 screws and tight the nuts. Since the holes on the metal plates are bigger than screws you can use washers now. Mount the PCB plates on the vertical holes on the box. Mount the lamp on the metal plates, to fit the lamp connectors you can now slide the plates in and out because the plate holes are lot bigger than the screws, once find the right setting (lamp should not be loose) tight the nuts and solder the banana connectors to the plates. Check the lamp if it works.

	=====Fiber outlet adapter=====		=====Fiber outlet adapter=====

Pranav Rathi: /* Lamp Mount */

Pranav Rathi — Fri, 25 Jan 2013 23:31:48 GMT

Lamp Mount

←Older revision		Revision as of 23:31, 25 January 2013
Line 101:		Line 101:

	=====Lamp Mount=====		=====Lamp Mount=====
-	This is the trickiest and most important part. This part depends on what materials you use, if you ceramic besides PCB then the procedure would be little different. I used PCB because the ceramic was not available in my lab. Get a plane PCB plates cut them in size of 1.5X2 inches get 6 of them and stack them together; slide 21 and 22. Make 3/8’’ through holes in all the plates on long side to align the holes made on the box on lamp side vertically. Prepare two peace of 1/4'' thick metal (choose you own dimensions) as shown in the slide 22. Make 2 3/8'' holes on each side of each plate. Drill one through hole on each plate to fit the lamp connector pins (this is important to be very careful). Take 3 PCB plates and make 4 4/40’’ through holes to align the 4 holes on the 2 metal plates. Take 2 PCB plates and make 4 4/40 screw’s head size holes to fit the 4/40 screw heads. Leave the left over PCB plate alone. Now stack them in this order: put 4/40 screws in 3 (4/40’’ size hole) PCB plates in front, 2 (4/40’’ screw‘s head size) plate after that and the leftover PCB plate in the end. Now put the metal plates on the 4 screws and tight the nuts. Since the holes on the metal plates are bigger than screws you can use ~~washer~~ now. Mount the PCB plates on the vertical holes on the box. Mount the lamp on the metal plates to fit the lamp connectors you can now slide the plates in and out because the plate holes are lot bigger than the screws, once find the right setting (lamp should not be loose) tight the nuts and solder the banana connectors to the plates. Check the lamp if it works.	+	This is the trickiest and most important part. This part depends on what materials you use, if you ceramic besides PCB then the procedure would be little different. I used PCB because the ceramic was not available in my lab. Get a plane PCB plates cut them in size of 1.5X2 inches get 6 of them and stack them together; slide 21 and 22. Make 3/8’’ through holes in all the plates on long side to align the holes made on the box-wall on lamp side vertically. Prepare two peace of 1/4 inch thick metal (choose you own dimensions) as shown in the slide 22. Make 2 3/8'' holes on each side of each plate. Drill one through hole on each plate to fit the lamp connector pins (this is important to be very careful). Take 3 PCB plates and make 4 4/40’’ through holes to align the 4 holes on the 2 metal plates. Take 2 PCB plates and make 4 4/40 screw’s head size holes to fit the 4/40 screw heads. Leave the left over PCB plate alone. Now stack them in this order: put 4/40 screws in 3 (4/40’’ size hole) PCB plates in front, 2 (4/40’’ screw‘s head size) plate after that and the leftover PCB plate in the end. Now put the metal plates on the 4 screws and tight the nuts. Since the holes on the metal plates are bigger than screws you can use washers now. Mount the PCB plates on the vertical holes on the box. Mount the lamp on the metal plates, to fit the lamp connectors you can now slide the plates in and out because the plate holes are lot bigger than the screws, once find the right setting (lamp should not be loose) tight the nuts and solder the banana connectors to the plates. Check the lamp if it works.

	=====Fiber outlet adapter=====		=====Fiber outlet adapter=====