User:Pranav Rathi/Notebook/OT/2011/10/11/Device for studying acoustic and mechanical noise in optical setups: Difference between revisions

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==Introduction==
==Introduction==
All optical setups are subjected to the mechanical and airborne acoustic noise generated by various electronic and mechanical devices such as computers and air-vents in a lab. Optical setups such as optical tweezers are very sensitive to these noises.  Optical tweezers like ours is so sensitive that it almost woke like a microphone.  Currently it is reading all kinds of mechanical and airborne noises which are very troublesome for DNA unzipping and overstretching experiments. So I had to do a full scale investigation of these noises[[http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2011/06/21/Noise_issue_with_the_optical_tweezers]]. To do it I had to create this device: I named it Acoustic & Mechanical Noise Reader (AMNR). This device is nothing but a condenser microphone inside the ear-plug of a stethoscope.  But it is so sensitive that it can read the mechanical noise up to -95DB (believe it or not), and it just uses a regular computer sound card with a Lab View V9 program. This whole setup has three parts; AMNR, noise investigator and helper program and, tone generator and speaker.
The whole purpose of this device is following:
*Identify the airborne noise frequencies around the tweezers and their source.
*Identify the mechanical noise (vibrations) frequencies in the optical table and their source.
*Help me chose and design the right setup (stages and sample holders) on the microscope.
*Help me in designing and searching ways to isolate the optical trap form acoustic and mechanical noise.
Most of this I discussed in the “Noise issue with the optical tweezers’’ page of my note book[[http://openwetware.org/wiki/User:Pranav_Rathi/Notebook/OT/2011/06/21/Noise_issue_with_the_optical_tweezers]]. In this page I will just discuss the construction and demonstration briefly.
==Hardware==
==Hardware==
==Construction==
==Construction==

Revision as of 12:25, 19 October 2011

Introduction

All optical setups are subjected to the mechanical and airborne acoustic noise generated by various electronic and mechanical devices such as computers and air-vents in a lab. Optical setups such as optical tweezers are very sensitive to these noises. Optical tweezers like ours is so sensitive that it almost woke like a microphone. Currently it is reading all kinds of mechanical and airborne noises which are very troublesome for DNA unzipping and overstretching experiments. So I had to do a full scale investigation of these noises[[1]]. To do it I had to create this device: I named it Acoustic & Mechanical Noise Reader (AMNR). This device is nothing but a condenser microphone inside the ear-plug of a stethoscope. But it is so sensitive that it can read the mechanical noise up to -95DB (believe it or not), and it just uses a regular computer sound card with a Lab View V9 program. This whole setup has three parts; AMNR, noise investigator and helper program and, tone generator and speaker.

The whole purpose of this device is following:

  • Identify the airborne noise frequencies around the tweezers and their source.
  • Identify the mechanical noise (vibrations) frequencies in the optical table and their source.
  • Help me chose and design the right setup (stages and sample holders) on the microscope.
  • Help me in designing and searching ways to isolate the optical trap form acoustic and mechanical noise.


Most of this I discussed in the “Noise issue with the optical tweezers’’ page of my note book[[2]]. In this page I will just discuss the construction and demonstration briefly.

Hardware

Construction

Demonstration