User:Pranav Rathi/Notebook/OT/2011/01/10/DNA tethering experiments: Difference between revisions
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Pranav Rathi (talk | contribs) (New page: I attempt tethering again today with new beads and new anti-dig. ===Ant-dig=== Dr. Koch and I prepared new anti-dig: 1X concentration *PBS [http://en.wikipedia.org/wiki/Phosphate_buffered...) |
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# Flow BGB 50μl + seal it with nail polish | # Flow BGB 50μl + seal it with nail polish | ||
===Results=== | ===Results=== | ||
[http://www.evernote.com/pub/pranavrathi1/dnastretching] | |||
{{#widget:YouTube|id=OvuG6Ju77h0}} | {{#widget:YouTube|id=OvuG6Ju77h0}} | ||
{{#widget:YouTube|id=A_CUeRuwxco}} | {{#widget:YouTube|id=A_CUeRuwxco}} | ||
{{#widget:YouTube|id=ehFeJ165Tis}} | {{#widget:YouTube|id=ehFeJ165Tis}} | ||
Revision as of 21:51, 10 January 2011
I attempt tethering again today with new beads and new anti-dig.
Ant-dig
Dr. Koch and I prepared new anti-dig: 1X concentration
- PBS [1]180μl + aliquots 20μl = [MIX] = Anti-dig 200μl.
PBS was used from the glass door fridge and aliquot from super cold gray fridge. I used two different beads:
- 1 μm new beads (looks good in microscope), 20μl from the batch. 5μl (batch) +4 5μl BGB = 50μl.
- .5μm (FL) expired on 01/07/2010.5μl (batch)+45μl BGB = 50μl.
Procedure[2]
- Flow anti-dig 10μl wait for 5 min.
- Flow BGB 50μl wait for 2 min.
- Flow 1.1kb (new)10μl wait for 14 min.
- Flow BGB wait none.
- Flow micro-spheres one in each chamber 10μl wait for 14 min.
- Flow BGB 50μl + seal it with nail polish
Results
{{#widget:YouTube|id=OvuG6Ju77h0}} {{#widget:YouTube|id=A_CUeRuwxco}} {{#widget:YouTube|id=ehFeJ165Tis}}
