User:Pranav Rathi/Notebook/OT/2010/12/10/Olympus Water Immersion Specs: Difference between revisions
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===Resolution and | ===Resolution and achievable spot size=== | ||
Revision as of 12:06, 13 December 2010
Water immersion objective details
WE are using Olympus UPLANSAPO (UIS 2) water immersion IR objective for DNA stretching and unzipping. The detail specifications of the objective can be found in the link:[1] Other specification are as follows;
| Mag | 60X |
| Wavelength | 1064 |
| NA | 1.2 |
| medium | water |
| Max ray angle | 64.5degrees |
| f # | 26.5 |
| Effective FL in water | 1.5 to 1.6mm (distance between the focal spot and the exit aperture surface) |
| Entrance aperture diameter | 8.5mm |
| Exit aperture diameter | 6.6mm |
| Working distance | .28mm |
| Cover glass correction | .13 to .21 (we use .15) |
