User:Paul Rothenberg/Notebook/Pauls Notebook/2014/07/07: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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From the timed run, here's the graph: | From the timed run, here's the graph: | ||
[[Image: | [[Image:Mb_Phosphate_OPD_H2O2_EtOH_25C_Chart_Corrected.png|500px]] | ||
Here are the charts from the control reactions: | Here are the charts from the control reactions: | ||
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[[Image:Propanol_Control_Reaction_120Min_Chart.png|500px]] | [[Image:Propanol_Control_Reaction_120Min_Chart.png|500px]] | ||
[[Image: | [[Image:THF_Control_Reaction_120Min_Chart.png|500px]] | ||
The propanol will definitely need to be run over time to see the reaction progression. | |||
Latest revision as of 00:04, 27 September 2017
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Further Control ReactionsRan two more control reactions today, in 1-propanol and THF. We also ran the Mb Phosphate in ethanol at 25°C over 2 hours in Ocean Optics. Procedure 10 mg/mL of Mb Phosphate and Citrate were made in each solvent, along with minimal hemin in 5 mL of solvent. UV-VIS was run to determine concentration. The dilutions were done as follows:
The OPD-Peroxide reactions were set up in 4 reactions per solvent. These were shaken over two hours, and UVVIS was taken of each reaction. Results From the timed run, here's the graph: Here are the charts from the control reactions: The propanol will definitely need to be run over time to see the reaction progression.
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