User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/18: Difference between revisions
From OpenWetWare
Line 8: | Line 8: | ||
==Reconstitution of Apoprotein== | ==Reconstitution of Apoprotein== | ||
Going to reconstitute the Apoprotein with MnPPIX. Hopefully the yield will be greater today. Before that, I ran a | Going to reconstitute the Apoprotein with MnPPIX. Hopefully the yield will be greater today. Before that, I ran a UV-VIS spec of the apoprotein at 1/20 and pure to compare concentration values. It looks like we are losing product during dialysis. | ||
Procedure for Today: | |||
To synthesize more apoprotein, we made a stock solution of 50 mM Tris buffer at pH 7.2. We dissolved 50 mg of myoglobin into 20 mL of buffer, and placed it on ice. The pH was brought to 2.5 at 0°C with the addition of 3.2 mL of 1 N HCl. | |||
Revision as of 07:30, 18 June 2014
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Reconstitution of ApoproteinGoing to reconstitute the Apoprotein with MnPPIX. Hopefully the yield will be greater today. Before that, I ran a UV-VIS spec of the apoprotein at 1/20 and pure to compare concentration values. It looks like we are losing product during dialysis.
|