User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/09: Difference between revisions
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==Preparation of more Apo-Mb== | ==Preparation of more Apo-Mb== | ||
Andrew and I are going to synthesize more Apo-Mb for preparation of Co-Mb. We will follow the same protocol as | Andrew and I are going to synthesize more Apo-Mb for preparation of Co-Mb. We will follow the same protocol as I did [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2014/03/05 here]. We extracted about 7 mL of apoprotein. We also had to mix pH 2 H<sub>2</sub>O with equal parts 2-butanone because solid clumps blocked the separatory funnel. We added 4 mL of each for a total volume of 16 mL before separation. We added the apoprotein to a dialysis cassette and let run overnight. | ||
==Synthesis of Co-Mb== | ==Synthesis of Co-Mb== | ||
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Preparation of more Apo-MbAndrew and I are going to synthesize more Apo-Mb for preparation of Co-Mb. We will follow the same protocol as I did here. We extracted about 7 mL of apoprotein. We also had to mix pH 2 H2O with equal parts 2-butanone because solid clumps blocked the separatory funnel. We added 4 mL of each for a total volume of 16 mL before separation. We added the apoprotein to a dialysis cassette and let run overnight. Synthesis of Co-Mb | |
