User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/02: Difference between revisions
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I am following the same procedure as [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2014/03/05| here] to prepare Apomyoglobin. | I am following the same procedure as [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2014/03/05| here] to prepare Apomyoglobin. | ||
The heme was extracted using the acid and 2-butanone method. Approximately 10 mL of the aqueous layer was produced. It was left to run dialysis overnight at 4°C in the 10 mM Na<sub>2</sub>CO<sub>3</sub> buffer. | |||
==Synthesis of Cobalt PPIX== | ==Synthesis of Cobalt PPIX== | ||
Revision as of 10:58, 2 June 2014
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Preparation of Apo-MbI am following the same procedure as here to prepare Apomyoglobin. The heme was extracted using the acid and 2-butanone method. Approximately 10 mL of the aqueous layer was produced. It was left to run dialysis overnight at 4°C in the 10 mM Na2CO3 buffer. Synthesis of Cobalt PPIX | |
