User:Paul Rothenberg/Notebook/Pauls Notebook/2014/04/16: Difference between revisions

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Latest revision as of 23:53, 26 September 2017

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Mb Citrate in H2O at 25°C

  1. Ran another Mb reaction, with 2.5 mg of OPD in 2.5 mL of H2O and 1.6 mg of Mb Citrate in 1 mL of H20.
  2. 2.025 mL of OPD solution and 0.75 mL of 30% H2O2 were added to the cuvette
  3. The cuvette was placed in the temperature controller and let sit
  4. The Mb was added at 1 min 45 s, and the reaction was run for 2 hours