User:Paul Rothenberg/Notebook/Pauls Notebook/2014/04/02: Difference between revisions
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== | ==Protein Purification== | ||
* | |||
*Today, I am going to run the first part of protein reconstitution on the synthesized Mn PPIX myoglobin. The procedure for today is as follows: | |||
#The column had ethanol present, and it was run out | |||
#15 mL of H<sub>2</sub>O was then run through it in 3x4 mL aliquots | |||
#25 mL of phosphate buffer was added to equilibrate the column | |||
#Once the column was equilibrated, 2.5 mL of the reaction mixture was added, and the waste container removed | |||
#3.5 mL of buffer was added, and the product was collected | |||
#25 mL of buffer was added to reequilibrate the column, and the procedure was repeated for the rest of the reaction mixture | |||
*100 mM Phosphate Buffer, 200 mL: | |||
#0.0083 mol acid component | |||
#0.0116 mol basic component | |||
#200 mL H<sub>2</sub>O | |||
Revision as of 06:55, 2 April 2014
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Protein Purification
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