User:Paul Rothenberg/Notebook/Pauls Notebook/2014/03/26: Difference between revisions
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==Reconstitution of Apoprotein== | ==Reconstitution of Apoprotein== | ||
*In order to reconstitute the apoprotein with the Mn PPIX, the ratio used in other syntheses was 2 mg PPIX per 10 mg apoprotein product (assuming full yield); this is still an excess of PPIX. For this reaction, 200 mg of myoglobin was used, so the stoichiometric amount of PPIX is 40 mg. Of which, we have 500 mg. | |||
*Next, we need 2 mL of 100mM Phosphate buffer at pH 7.0, and 0.1 N (0.1 M) NaOH. | |||
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Revision as of 07:29, 26 March 2014
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UV-VIS of Apoprotein
Reconstitution of Apoprotein
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