User:Paul Rothenberg/Notebook/Pauls Notebook/2014/03/26

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(UV-VIS of Apoprotein)
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==UV-VIS of Apoprotein==
==UV-VIS of Apoprotein==
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* UV-VIS spectroscopy was run on the protein to determine if the extraction of the heme groups was successful. If so, there should be a peak at 280 nm. If there is heme left, there would be a peak at 400 nm.  
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* UV-VIS spectroscopy was run on the protein to determine if the extraction of the heme groups was successful. If so, there should be a peak at 280 nm. If there is heme left, there would be a peak at 400 nm.
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*The spectrum revealed a large peak at 280 nm (roughly 0.7) and a very slight peak at 400 (0.06-0.07).
==Reconstitution of Apoprotein==
==Reconstitution of Apoprotein==

Revision as of 09:23, 26 March 2014

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UV-VIS of Apoprotein

  • UV-VIS spectroscopy was run on the protein to determine if the extraction of the heme groups was successful. If so, there should be a peak at 280 nm. If there is heme left, there would be a peak at 400 nm.
  • The spectrum revealed a large peak at 280 nm (roughly 0.7) and a very slight peak at 400 (0.06-0.07).

Reconstitution of Apoprotein


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