User:Paul Rothenberg/Notebook/Pauls Notebook/2014/03/05

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(Heme Extraction from Myoglobin)
(Heme Extraction from Myoglobin)
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==Heme Extraction from Myoglobin==
==Heme Extraction from Myoglobin==
* Preparation of Apoprotein:
* Preparation of Apoprotein:
-
#24.228 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 1 gram of myoglobin
+
#24.3 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 200 mg of myoglobin
 +
# 3.36 g of Sodium Bicarbonate was dissolved in 4L of H<sub>2</sub>O to make a 10 mM dialysis buffer
 +
#

Revision as of 10:19, 5 March 2014

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Heme Extraction from Myoglobin

  • Preparation of Apoprotein:
  1. 24.3 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 200 mg of myoglobin
  2. 3.36 g of Sodium Bicarbonate was dissolved in 4L of H2O to make a 10 mM dialysis buffer



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