User:Paul Rothenberg/Notebook/Pauls Notebook/2013/07/01: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 17: Line 17:
== Synthesis of Buffered Mb ==
== Synthesis of Buffered Mb ==


This synthesis was taken from [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/22 May 22].


5 mg of Mb and approximately 490-500 milligrams of KCl were added to four tubes, and 20 mL of pH 3 Citrate, pH 5 Acetate, pH 7 Phosphate, and pH 9 Tris buffers were added.


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 06:33, 1 July 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Reaction of OPD+H2O2 with Mb/Acetate Catalyst in H2O

Going to continue the trials in H2O with Mb/Acetate. We are also going to make up more buffered protein solutions.

Trial 2 Data:


Trial 3 Data:

Synthesis of Buffered Mb

This synthesis was taken from May 22.

5 mg of Mb and approximately 490-500 milligrams of KCl were added to four tubes, and 20 mL of pH 3 Citrate, pH 5 Acetate, pH 7 Phosphate, and pH 9 Tris buffers were added.