User:Paul Rothenberg/Notebook/Pauls Notebook/2013/07/01

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(Reaction of OPD+H2O2 with Mb/Acetate Catalyst in H2O)
Current revision (11:08, 8 July 2013) (view source)
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Going to continue the trials in H<sub>2</sub>O with Mb/Acetate. We are also going to make up more buffered protein solutions.  
Going to continue the trials in H<sub>2</sub>O with Mb/Acetate. We are also going to make up more buffered protein solutions.  
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<b>Trial 2 Data:</sub>
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<b>Trial 2 Data:</b>
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[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_GRAPH_Trial2.png|750px]]
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<b>Trial 3 Data:</sub>
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[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_SEQUENTIAL_GRAPH_Trial2.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_GRAPH_Trial2.png|750px]]
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 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_SEQUENTIAL_GRAPH_Trial2.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_GRAPH_Trial2.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_SEQUENTIAL_GRAPH_Trial2.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_GRAPH_Trial2.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_SEQUENTIAL_GRAPH_Trial2.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_GRAPH_Trial2.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_SEQUENTIAL_GRAPH_Trial2.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_GRAPH_Trial2.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_SEQUENTIAL_GRAPH_Trial2.png|750px]]
 +
 
 +
<b>Trial 3 Data:</b>
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_65C_GRAPH_Trial3.png|750px]]
 +
 
 +
[[Image:Mb_Acetate_OPD_H2O2_H2O_65C_SEQUENTIAL_GRAPH_Trial3.png|750px]]
== Synthesis of Buffered Mb ==
== Synthesis of Buffered Mb ==
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This synthesis was taken from [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/22 May 22].
 +
5 mg of Mb and approximately 490-500 milligrams of KCl were added to four tubes, and 20 mL of pH 3 Citrate, pH 5 Acetate, pH 7 Phosphate, and pH 9 Tris buffers were added.
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Reaction of OPD+H2O2 with Mb/Acetate Catalyst in H2O

Going to continue the trials in H2O with Mb/Acetate. We are also going to make up more buffered protein solutions.

Trial 2 Data:

Trial 3 Data:

Synthesis of Buffered Mb

This synthesis was taken from May 22.

5 mg of Mb and approximately 490-500 milligrams of KCl were added to four tubes, and 20 mL of pH 3 Citrate, pH 5 Acetate, pH 7 Phosphate, and pH 9 Tris buffers were added.


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